≡

Splicing Factor 3B Subunit 2 (SF3B2)

[Edit]

SAP145; SF3B145; SF3b1; SF3b150; Spliceosome-associated protein 145; Pre-mRNA-splicing factor SF3b 145 kDa subunit

Splicing Factor 3B Subunit 2 (SF3B2)
CCC M215
HGNC 10769
MGI 2441856
PubMed 22415777
RGD 1307159
UniProt Q13435
Wiki SF3B2
SF3B2 encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA.
Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Splicing Factor 3B Subunit 2 (SF3B2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Splicing Factor 3B Subunit 2 (SF3B2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Splicing Factor 3B Subunit 2 (SF3B2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Splicing Factor 3B Subunit 2 (SF3B2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Splicing Factor 3B Subunit 2 (SF3B2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Splicing Factor 3B Subunit 2 (SF3B2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Splicing Factor 3B Subunit 2 (SF3B2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Splicing Factor 3B Subunit 2 (SF3B2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Splicing Factor 3B Subunit 2 (SF3B2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Splicing Factor 3B Subunit 2 (SF3B2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Splicing Factor 3B Subunit 2 (SF3B2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Splicing Factor 3B Subunit 2 (SF3B2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Splicing Factor 3B Subunit 2 (SF3B2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Splicing Factor 3B Subunit 2 (SF3B2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Splicing Factor 3B Subunit 2 (SF3B2) ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A."Genes Dev. 10:233-243(1996) [PubMed] [Europe PMC] [Abstract]
  4. "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E."Mol. Cell 5:779-787(2000) [PubMed] [Europe PMC] [Abstract]
  5. "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein."EMBO J. 21:4978-4988(2002) [PubMed] [Europe PMC] [Abstract]
  6. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
  7. "Molecular architecture of the multiprotein splicing factor SF3b."Science 300:980-984(2003) [PubMed] [Europe PMC] [Abstract]
  8. "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome."RNA 10:929-941(2004) [PubMed] [Europe PMC] [Abstract]
  9. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Human immunodeficiency virus type 1 Vpr induces G2 checkpoint activation by interacting with the splicing factor SAP145."Mol. Cell. Biol. 26:8149-8158(2006) [PubMed] [Europe PMC] [Abstract]
  11. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  12. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  13. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  14. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  19. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  20. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  21. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  22. "Solution structure of the SAP domain of human splicing factor 3B subunit 2."Submitted (APR-2007) to the PDB data bank