≡

O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS)

[Edit]

SLA/LP; SLA; SLA-p35; Sep tRNA:Sec tRNA Synthase; Selenocysteinyl-tRNA(Sec) synthase; Selenocysteine synthase; Soluble Liver Antigen/Liver Pancreas Antigen

O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS)
CCC M206
HGNC 30605
MGI 1098791
PubMed 19608919
UniProt Q9HD40
Wiki SEPSECS
Autoantibodies to soluble liver antigen (SLA) and to liver and pancreas antigen (LP) occur in about 30% of all patients with autoimmune hepatitis.
The 21st amino acid, selenocysteine (sec), is distinct from other amino acids because it lacks its own tRNA synthetase and is the only amino acid synthesized on its cognate tRNA. Synthesis of sec begins with acylation of tRNA(sec) (TRSP) by seryl-tRNA synthetase (SARS) to give ser-tRNA(sec), which is subsequently phosphorylated by O-phosphoseryl-tRNA kinase (PSTK) to give O-phosphoseryl-tRNA(sec). SEPSECS catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS) CLIA Kit Customized Service Offer
n/a ELISA Kit for O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS) CLIA Kit Customized Service Offer
n/a ELISA Kit for O-Phosphoserine tRNA Selenocysteine tRNA Synthase (SEPSECS) ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Identification of target antigen for SLA/LP autoantibodies in autoimmune hepatitis."Lancet 355:1510-1515(2000) [PubMed] [Europe PMC] [Abstract]
  6. "Isolation and characterization of cDNA encoding the antigenic protein of the human tRNP(Ser)Sec complex recognized by autoantibodies from patients withtype-1 autoimmune hepatitis."Clin. Exp. Immunol. 121:364-374(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Soluble liver antigen: isolation of a 35-kd recombinant protein (SLA-p35) specifically recognizing sera from patients with autoimmune hepatitis."Hepatology 33:591-596(2001) [PubMed] [Europe PMC] [Abstract]
  8. "Fine specificity of autoantibodies to soluble liver antigen and liver/pancreas."Hepatology 35:403-408(2002) [PubMed] [Europe PMC] [Abstract]
  9. "RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea."Proc. Natl. Acad. Sci. U.S.A. 103:18923-18927(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  12. "The human SepSecS-tRNASec complex reveals the mechanism of selenocysteine formation."Science 325:321-325(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Mutations disrupting selenocysteine formation cause progressive cerebello-cerebral atrophy."Am. J. Hum. Genet. 87:538-544(2010) [PubMed] [Europe PMC] [Abstract]