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SUMO Activating Enzyme Subunit 1 (SAE1)

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AOS1; SUA1; UBLE1A; Activator Of Sumo 1; Ubiquitin-like 1-activating enzyme E1A

SUMO Activating Enzyme Subunit 1 (SAE1)
CCC M179
HGNC 30660
MGI 1929264
PubMed 10187858
RGD 1306098
UniProt Q9UBE0
Wiki SAE1
Posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.The deduced SAE1 and SAE2 proteins contain 347 and 640 amino acids, respectively.
SAE1 shares sequence similarity with the N terminus of ubiquitin-activating E1 enzymes, and SAE2 share sequence similarity with the C terminus of E1 enzymes. Both SAE subunits contain a conserved nucleotide-binding motif, and SAE2 contains an E1-like active-site cysteine. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SUMO Activating Enzyme Subunit 1 (SAE1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SUMO Activating Enzyme Subunit 1 (SAE1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SUMO Activating Enzyme Subunit 1 (SAE1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SUMO Activating Enzyme Subunit 1 (SAE1) CLIA Kit Customized Service Offer
n/a ELISA Kit for SUMO Activating Enzyme Subunit 1 (SAE1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SUMO Activating Enzyme Subunit 1 (SAE1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SUMO Activating Enzyme Subunit 1 (SAE1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SUMO Activating Enzyme Subunit 1 (SAE1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SUMO Activating Enzyme Subunit 1 (SAE1) CLIA Kit Customized Service Offer
n/a ELISA Kit for SUMO Activating Enzyme Subunit 1 (SAE1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SUMO Activating Enzyme Subunit 1 (SAE1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SUMO Activating Enzyme Subunit 1 (SAE1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SUMO Activating Enzyme Subunit 1 (SAE1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SUMO Activating Enzyme Subunit 1 (SAE1) CLIA Kit Customized Service Offer
n/a ELISA Kit for SUMO Activating Enzyme Subunit 1 (SAE1) ELISA Kit Customized Service Offer
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  2. "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex."FEBS Lett. 448:185-189(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1."J. Biol. Chem. 274:10618-10624(1999) [PubMed] [Europe PMC] [Abstract]
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  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Expression and regulation of the mammalian SUMO-1 E1 enzyme."FASEB J. 15:1825-1827(2001) [PubMed] [Europe PMC] [Abstract]
  9. "Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9."J. Biol. Chem. 276:35368-35374(2001) [PubMed] [Europe PMC] [Abstract]
  10. "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers."Arch. Biochem. Biophys. 453:70-74(2006) [PubMed] [Europe PMC] [Abstract]
  11. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  13. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  15. "Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1."EMBO J. 24:439-451(2005) [PubMed] [Europe PMC] [Abstract]
  16. "Active site remodelling accompanies thioester bond formation in the SUMO E1."Nature 463:906-912(2010) [PubMed] [Europe PMC] [Abstract]