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Nth Endonuclease III Like Protein 1 (NTHL1)

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NTH1; OCTS3; Endonuclease III-like protein 1; Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase

Nth Endonuclease III Like Protein 1 (NTHL1)
CCC L973
HGNC 8028
MGI 1313275
PubMed 9831664
RGD 1309289
UniProt P78549
Wiki NTHL1
DNA N-glycosylases, or apurinic/apyrimidinic lyases, of the endonuclease III family, such as NTHL1, initiate DNA base excision repair of oxidized ring saturated pyrimidine residues.
The human NTH1 gene encodes a 312-amino acid polypeptide with a predicted mass of 34.3 kD. When expressed and purified, the protein had the same enzymatic activities as the E. coli enzyme: DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues, and apurinic/apyrimidinic lyase activity. NTHL1 contains 6 exons spanning approximately 8 kb of genomic DNA. Promoter analysis indicated a lack of TATA and CAAT boxes but the presence of a CpG island with binding sites for several transcription factors.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Nth Endonuclease III Like Protein 1 (NTHL1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nth Endonuclease III Like Protein 1 (NTHL1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nth Endonuclease III Like Protein 1 (NTHL1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nth Endonuclease III Like Protein 1 (NTHL1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nth Endonuclease III Like Protein 1 (NTHL1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Nth Endonuclease III Like Protein 1 (NTHL1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nth Endonuclease III Like Protein 1 (NTHL1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nth Endonuclease III Like Protein 1 (NTHL1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nth Endonuclease III Like Protein 1 (NTHL1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nth Endonuclease III Like Protein 1 (NTHL1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Nth Endonuclease III Like Protein 1 (NTHL1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nth Endonuclease III Like Protein 1 (NTHL1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nth Endonuclease III Like Protein 1 (NTHL1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nth Endonuclease III Like Protein 1 (NTHL1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nth Endonuclease III Like Protein 1 (NTHL1) ELISA Kit Customized Service Offer
  1. "Cloning and characterization of a functional human homolog of Escherichia coli endonuclease III."Proc. Natl. Acad. Sci. U.S.A. 94:109-114(1997) [PubMed] [Europe PMC] [Abstract]
  2. "Genomic structure and sequence of a human homologue (NTHL1/NTH1) of Escherichia coli endonuclease III with those of the adjacent parts of TSC2 and SLC9A3R2 genes."Gene 222:287-295(1998) [PubMed] [Europe PMC] [Abstract]
  3. "The sequence and analysis of duplication-rich human chromosome 16." Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue."J. Biol. Chem. 273:21585-21593(1998) [PubMed] [Europe PMC] [Abstract]
  5. "Cloning and expression of the cDNA encoding the human homologue of the DNA repair enzyme, Escherichia coli endonuclease III."J. Biol. Chem. 272:6733-6740(1997) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Cell-cycle regulation, intracellular sorting and induced overexpression of the human NTH1 DNA glycosylase involved in removal of formamidopyrimidine residues from DNA."Mutat. Res. 460:95-104(2000) [PubMed] [Europe PMC] [Abstract]
  8. "Truncation of amino-terminal tail stimulates activity of human endonuclease III (hNTH1)."J. Mol. Biol. 321:265-276(2002) [PubMed] [Europe PMC] [Abstract]
  9. "Mitochondrial targeting of human DNA glycosylases for repair of oxidative DNA damage."Nucleic Acids Res. 26:2917-2922(1998) [PubMed] [Europe PMC] [Abstract]
  10. "Excision of products of oxidative DNA base damage by human NTH1 protein."Biochemistry 38:243-246(1999) [PubMed] [Europe PMC] [Abstract]
  11. "Human endonuclease III acts preferentially on DNA damage opposite guanine residues in DNA."Biochemistry 40:6653-6659(2001) [PubMed] [Europe PMC] [Abstract]
  12. "Mutation at active site lysine 212 to arginine uncouples the glycosylase activity from the lyase activity of human endonuclease III."Biochemistry 40:13617-13622(2001) [PubMed] [Europe PMC] [Abstract]
  13. "Stimulation of human endonuclease III by Y box-binding protein 1 (DNA-binding protein B). Interaction between a base excision repair enzyme and a transcription factor."J. Biol. Chem. 276:21242-21249(2001) [PubMed] [Europe PMC] [Abstract]
  14. "Escherichia coli Nth and human hNTH1 DNA glycosylases are involved in removal of 8-oxoguanine from 8-oxoguanine/guanine mispairs in DNA."Nucleic Acids Res. 29:1975-1981(2001) [PubMed] [Europe PMC] [Abstract]
  15. "Differential intracellular localization of the human and mouse endonuclease III homologs and analysis of the sorting signals."DNA Repair 1:847-854(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Identification of 5-formyluracil DNA glycosylase activity of human hNTH1 protein."Nucleic Acids Res. 30:3443-3448(2002) [PubMed] [Europe PMC] [Abstract]
  17. "Substrate specificity of human endonuclease III (hNTH1). Effect of human APE1 on hNTH1 activity."J. Biol. Chem. 278:9005-9012(2003) [PubMed] [Europe PMC] [Abstract]
  18. "Differential specificity of human and Escherichia coli endonuclease III and VIII homologues for oxidative base lesions."J. Biol. Chem. 279:14464-14471(2004) [PubMed] [Europe PMC] [Abstract]
  19. "DNA glycosylase activities for thymine residues oxidized in the methyl group are functions of the hNEIL1 and hNTH1 enzymes in human cells."DNA Repair 4:71-79(2005) [PubMed] [Europe PMC] [Abstract]
  20. "Initiation of base excision repair of oxidative lesions in nucleosomes by the human, bifunctional DNA glycosylase NTH1."Mol. Cell. Biol. 27:8442-8453(2007) [PubMed] [Europe PMC] [Abstract]
  21. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Non-specific DNA binding interferes with the efficient excision of oxidative lesions from chromatin by the human DNA glycosylase, NEIL1."DNA Repair 9:134-143(2010) [PubMed] [Europe PMC] [Abstract]
  23. "Fluorescent probes for the analysis of DNA strand scission in base excision repair."Nucleic Acids Res. 38:E101-E101(2010) [PubMed] [Europe PMC] [Abstract]
  24. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  25. "Nucleosome disruption by DNA ligase III-XRCC1 promotes efficient base excision repair."Mol. Cell. Biol. 31:4623-4632(2011) [PubMed] [Europe PMC] [Abstract]
  26. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  27. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]