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Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L)

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RSP5; hNedd4-2; E3 ubiquitin-protein ligase NEDD4-like

Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L)
CCC L912
HGNC 7728
MGI 1933754
PubMed 19917253
RGD 735047
UniProt Q96PU5
Wiki NEDD4L
The full-length cDNA sequence of 3,246 bp, obtained by RACE, contains an open reading frame of 2,562 nucleotides. The deduced 854-amino acid polypeptide was predicted to contain 4 WW domains and an HECT ubiquitin-protein ligase domain, highly conserved features in the NEDD4 gene family. The NEDD4L gene has 97% and 62% amino acid sequence identity to mouse Nedd4-2 and human NEDD4 genes, respectively. By expression analysis, a 3.4-kb band was observed in heart and muscle, while a 3.2-kb band and/or an additional 3.6-kb band were seen in other tissues examined. An alternative splicing event involving exon 12 of 60 bp was observed, the shorter allele being predominantly present in brain and lymphocytes, while the longer allele was strongly expressed in kidney and placenta.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) CLIA Kit Customized Service Offer
n/a ELISA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) CLIA Kit Customized Service Offer
n/a ELISA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) CLIA Kit Customized Service Offer
n/a ELISA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 4 Like Protein (NEDD4L) ELISA Kit Customized Service Offer
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  2. "Identification of new partners of the epithelial sodium channel alpha subunit."C. R. Biol. 326:615-624(2003) [PubMed] [Europe PMC] [Abstract]
  3. "Androgens differentially regulate the expression of NEDD4L transcripts in LNCaP human prostate cancer cells."Mol. Cell. Endocrinol. 210:51-62(2003) [PubMed] [Europe PMC] [Abstract]
  4. "DNA sequence and analysis of human chromosome 18." Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
  7. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  8. "14-3-3 proteins modulate the expression of epithelial Na+ channels by phosphorylation-dependent interaction with Nedd4-2 ubiquitin ligase."J. Biol. Chem. 280:13187-13194(2005) [PubMed] [Europe PMC] [Abstract]
  9. "Latent membrane protein 2A of Epstein-Barr virus binds WW domain E3 protein-ubiquitin ligases that ubiquitinate B-cell tyrosine kinases."Mol. Cell. Biol. 20:8526-8535(2000) [PubMed] [Europe PMC] [Abstract]
  10. "Serum and glucocorticoid-regulated kinase modulates Nedd4-2-mediated inhibition of the epithelial Na+ channel."J. Biol. Chem. 277:5-8(2002) [PubMed] [Europe PMC] [Abstract]
  11. "N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein."J. Biol. Chem. 277:9307-9317(2002) [PubMed] [Europe PMC] [Abstract]
  12. "Regulation of the glutamate transporter EAAT1 by the ubiquitin ligase Nedd4-2 and the serum and glucocorticoid-inducible kinase isoforms SGK1/3 and protein kinase B."J. Neurochem. 86:1181-1188(2003) [PubMed] [Europe PMC] [Abstract]
  13. "Cardiac voltage-gated sodium channel Nav1.5 is regulated by Nedd4-2 mediated ubiquitination."Circ. Res. 95:284-291(2004) [PubMed] [Europe PMC] [Abstract]
  14. "Nedd4-2 functionally interacts with ClC-5: involvement in constitutive albumin endocytosis in proximal tubule cells."J. Biol. Chem. 279:54996-55007(2004) [PubMed] [Europe PMC] [Abstract]
  15. "cAMP and serum and glucocorticoid-inducible kinase (SGK) regulate the epithelial Na(+) channel through convergent phosphorylation of Nedd4-2."J. Biol. Chem. 279:45753-45758(2004) [PubMed] [Europe PMC] [Abstract]
  16. "Regulation of the voltage gated K+ channel Kv1.3 by the ubiquitin ligase Nedd4-2 and the serum and glucocorticoid inducible kinase SGK1."J. Cell. Physiol. 199:194-199(2004) [PubMed] [Europe PMC] [Abstract]
  17. "Molecular determinants of voltage-gated sodium channel regulation by the Nedd4/Nedd4-like proteins."Am. J. Physiol. 288:C692-C701(2005) [PubMed] [Europe PMC] [Abstract]
  18. "NEDD4-2 (neural precursor cell expressed, developmentally down-regulated 4-2) negatively regulates TGF-beta (transforming growth factor-beta) signalling by inducing ubiquitin-mediated degradation of Smad2 and TGF-beta type I receptor."Biochem. J. 386:461-470(2005) [PubMed] [Europe PMC] [Abstract]
  19. "Nedd4-2 phosphorylation induces serum and glucocorticoid-regulated kinase (SGK) ubiquitination and degradation."J. Biol. Chem. 280:4518-4523(2005) [PubMed] [Europe PMC] [Abstract]
  20. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  21. "Nedd4 family-interacting protein 1 (Ndfip1) is required for the exosomal secretion of Nedd4 family proteins."J. Biol. Chem. 283:32621-32627(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  23. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  24. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  25. "Identification of NPC2 protein as interaction molecule with C2 domain of human Nedd4L."Biochem. Biophys. Res. Commun. 388:290-296(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Control of the activity of WW-HECT domain E3 ubiquitin ligases by NDFIP proteins."EMBO Rep. 10:501-507(2009) [PubMed] [Europe PMC] [Abstract]
  27. "Down-regulation of active ACK1 is mediated by association with the E3 ubiquitin ligase Nedd4-2."J. Biol. Chem. 284:8185-8194(2009) [PubMed] [Europe PMC] [Abstract]
  28. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  29. "Serum and glucocorticoid-induced kinase (SGK) 1 and the epithelial sodium channel are regulated by multiple with no lysine (WNK) family members."J. Biol. Chem. 285:25161-25167(2010) [PubMed] [Europe PMC] [Abstract]
  30. "HECT E3 ubiquitin ligase Nedd4-1 ubiquitinates ACK and regulates epidermal growth factor (EGF)-induced degradation of EGF receptor and ACK."Mol. Cell. Biol. 30:1541-1554(2010) [PubMed] [Europe PMC] [Abstract]
  31. "Interaction of serum- and glucocorticoid regulated kinase 1 (SGK1) with the WW-domains of Nedd4-2 is required for epithelial sodium channel regulation."PLoS ONE 5:E12163-E12163(2010) [PubMed] [Europe PMC] [Abstract]
  32. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  33. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  34. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  35. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  36. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  37. "A naturally occurring human Nedd4-2 variant displays impaired ENaC regulation in Xenopus laevis oocytes."Am. J. Physiol. 287:F550-F561(2004) [PubMed] [Europe PMC] [Abstract]