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U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2)

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U2AF65; •U2 auxiliary factor 65 kDa subunit; U2 snRNP auxiliary factor large subunit; Splicing factor U2AF 65 kDa subunit

U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2)
CCC L885
HGNC 23156
MGI 98886
PubMed 23645565
UniProt P26368
Wiki U2AF2
U2 auxiliary factor (U2AF), composed of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 encodes the U2AF large subunit, which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple alternatively spliced transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.U2AF65 splicing factor undergoes posttranslational lysyl-5-hydroxylation catalyzed by the Fe(II)- and 2-oxoglutarate-dependent dioxygenase Jumonji domain-6 protein (JMJD6).

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2) CLIA Kit Customized Service Offer
n/a ELISA Kit for U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2) CLIA Kit Customized Service Offer
n/a ELISA Kit for U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF2) ELISA Kit Customized Service Offer
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  3. , Submitted (NOV-2006) to UniProtKB
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  7. "Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent protein kinase regulates spliceosome assembly."EMBO J. 18:4549-4559(1999) [PubMed] [Europe PMC] [Abstract]
  8. "Tau exon 10, whose missplicing causes frontotemporal dementia, is regulated by an intricate interplay of cis elements and trans factors."J. Neurochem. 88:1078-1090(2004) [PubMed] [Europe PMC] [Abstract]
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  10. "U2AF-homology motif interactions are required for alternative splicing regulation by SPF45."Nat. Struct. Mol. Biol. 14:620-629(2007) [PubMed] [Europe PMC] [Abstract]
  11. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  13. "The protein factors MBNL1 and U2AF65 bind alternative RNA structures to regulate splicing."Proc. Natl. Acad. Sci. U.S.A. 106:9203-9208(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Jmjd6 catalyses lysyl-hydroxylation of U2AF65, a protein associated with RNA splicing."Science 325:90-93(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  18. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  19. "SKIP counteracts p53-mediated apoptosis via selective regulation of p21Cip1 mRNA splicing."Genes Dev. 25:701-716(2011) [PubMed] [Europe PMC] [Abstract]
  20. "The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex."Genes Dev. 25:972-983(2011) [PubMed] [Europe PMC] [Abstract]
  21. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  22. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  23. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  24. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  25. "Solution structures of the first and second RNA-binding domains of human U2 small nuclear ribonucleoprotein particle auxiliary factor (U2AF(65))."EMBO J. 18:4523-4534(1999) [PubMed] [Europe PMC] [Abstract]
  26. "Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP."Mol. Cell 11:965-976(2003) [PubMed] [Europe PMC] [Abstract]
  27. "A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer."Cell 106:595-605(2001) [PubMed] [Europe PMC] [Abstract]