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Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP)

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GANP; MAP80; Germinal-center associated nuclear protein; 80 kDa MCM3-associated protein; MCM3 acetyltransferase; MCM3 acetylating protein

Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP)
CCC L717
HGNC 6946
MGI 1930089
PubMed 23094019
RGD 1306834
UniProt O60318
Wiki MCM3AP
MCM3APwas reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization.The predicted 721-amino acid MAP80 has an estimated pI of 5.1. Antibodies against MAP80 detected an 80-kD protein on Western blots of HeLa cell extracts. The authors found that MAP80 coimmunoprecipitated with MCM3 in HeLa cell extracts. In the presence of a small amount of cytosol, recombinant MAP80 facilitated the nuclear import of a chimeric GFP-MCM3 protein.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPL717Hu01 Recombinant Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Minichromosome Maintenance Deficient 3 Associated Protein (MCM3AP) ELISA Kit Customized Service Offer
  1. "Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication."Gene 255:219-227(2000) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence of human chromosome 21." Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract]
  5. "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization."J. Biol. Chem. 273:22177-22180(1998) [PubMed] [Europe PMC] [Abstract]
  6. "MCM3AP, a novel acetyltransferase that acetylates replication protein MCM3."EMBO Rep. 2:119-123(2001) [PubMed] [Europe PMC] [Abstract]
  7. "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3."J. Biol. Chem. 277:43121-43125(2002) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  11. "mRNA export from mammalian cell nuclei is dependent on GANP."Curr. Biol. 20:25-31(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  13. "MCM3AP is transcribed from a promoter within an intron of the overlapping gene for GANP."J. Mol. Biol. 406:355-361(2011) [PubMed] [Europe PMC] [Abstract]
  14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  15. "Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export."Nucleic Acids Res. 40:4562-4573(2012) [PubMed] [Europe PMC] [Abstract]
  16. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
  17. "Identification of pathogenic gene variants in small families with intellectually disabled siblings by exome sequencing." J. Med. Genet. 50:802-811(2013) [PubMed] [Europe PMC] [Abstract]