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HECT Domain And RCC1 Like Domain Protein 5 (HERC5)

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CEB1; CEBP1; E3 ISG15 protein ligase HERC5; Cyclin-E-binding protein 1

HECT Domain And RCC1 Like Domain Protein 5 (HERC5)
CCC L578
HGNC 24368
PubMed 16407192
UniProt Q9UII4
Wiki HERC5
HERC5 is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.
Immunoprecipitation and immunoblot analysis confirmed that HERC5 specifically copurified with ISG15. RT-PCR analysis detected upregulated HERC5 expression after IFNB treatment. Suppression of HERC5 using small interfering RNA showed that HERC5 was required for the ISG15 conjugation system (ISGylation) following IFNB treatment.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant HECT Domain And RCC1 Like Domain Protein 5 (HERC5) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to HECT Domain And RCC1 Like Domain Protein 5 (HERC5) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to HECT Domain And RCC1 Like Domain Protein 5 (HERC5) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for HECT Domain And RCC1 Like Domain Protein 5 (HERC5) CLIA Kit Customized Service Offer
n/a ELISA Kit for HECT Domain And RCC1 Like Domain Protein 5 (HERC5) ELISA Kit Customized Service Offer
  1. "A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins."Biochem. Biophys. Res. Commun. 266:115-122(1999) [PubMed] [Europe PMC] [Abstract]
  2. "HERC5, a HECT E3 ubiquitin ligase tightly regulated in LPS activated endothelial cells."J. Cell Sci. 117:4749-4756(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Identification and Herc5-mediated ISGylation of novel target proteins."Biochem. Biophys. Res. Commun. 348:473-477(2006) [PubMed] [Europe PMC] [Abstract]
  6. "Herc5, an interferon-induced HECT E3 enzyme, is required for conjugation of ISG15 in human cells."J. Biol. Chem. 281:4334-4338(2006) [PubMed] [Europe PMC] [Abstract]
  7. "HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets."Proc. Natl. Acad. Sci. U.S.A. 103:10735-10740(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Herc5 attenuates influenza A virus by catalyzing ISGylation of viral NS1 protein."J. Immunol. 184:5777-5790(2010) [PubMed] [Europe PMC] [Abstract]
  9. "Positive regulation of interferon regulatory factor 3 activation by Herc5 via ISG15 modification."Mol. Cell. Biol. 30:2424-2436(2010) [PubMed] [Europe PMC] [Abstract]
  10. "The ISG15 conjugation system broadly targets newly synthesized proteins: implications for the antiviral function of ISG15."Mol. Cell 38:722-732(2010) [PubMed] [Europe PMC] [Abstract]
  11. "ISG15 conjugation system targets the viral NS1 protein in influenza A virus-infected cells."Proc. Natl. Acad. Sci. U.S.A. 107:2253-2258(2010) [PubMed] [Europe PMC] [Abstract]