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Egl Nine Homolog 3 (EGLN3)

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HIFPH3; PHD3; HPH-1; HPH-3; HIF Prolyl Hydroxylase 3; Hypoxia-inducible factor prolyl hydroxylase 3; Prolyl hydroxylase domain-containing protein 3

Egl Nine Homolog 3 (EGLN3)
CCC L463
HGNC 14661
MGI 1932288
PubMed 16098468
RGD 71019
UniProt Q9H6Z9
Wiki EGLN3
HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. Posttranslational modification by prolyl hydroxylation is a key regulatory event that targets HIF-alpha (HIF1) subunits for proteasomal destruction via the von Hippel-Lindau (VHL) ubiquitylation complex. In mammalian cells, they showed that the HIF-prolyl hydroxylases are represented by 3 proteins with a conserved 2-histidine-1-carboxylate iron coordination motif at the catalytic site. The genes encoding these proteins were cloned and termed PHD1, PHD2, and PHD3 by the authors. Direct modulation of recombinant enzyme activity by graded hypoxia, iron chelation, and cobaltous ions mirrored the characteristics of HIF induction in vivo, fulfilling requirements for these enzymes being oxygen sensors that regulate HIF.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Egl Nine Homolog 3 (EGLN3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Egl Nine Homolog 3 (EGLN3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Egl Nine Homolog 3 (EGLN3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Egl Nine Homolog 3 (EGLN3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Egl Nine Homolog 3 (EGLN3) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Egl Nine Homolog 3 (EGLN3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Egl Nine Homolog 3 (EGLN3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Egl Nine Homolog 3 (EGLN3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Egl Nine Homolog 3 (EGLN3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Egl Nine Homolog 3 (EGLN3) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Egl Nine Homolog 3 (EGLN3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Egl Nine Homolog 3 (EGLN3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Egl Nine Homolog 3 (EGLN3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Egl Nine Homolog 3 (EGLN3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Egl Nine Homolog 3 (EGLN3) ELISA Kit Customized Service Offer
  1. "Characterization and comparative analysis of the EGLN gene family."Gene 275:125-132(2001) [PubMed] [Europe PMC] [Abstract]
  2. "Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-inducible factor."J. Biol. Chem. 278:30772-30780(2003) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation."Cell 107:43-54(2001) [PubMed] [Europe PMC] [Abstract]
  6. "HIF-1, O(2), and the 3 PHDs: how animal cells signal hypoxia to the nucleus."Cell 107:1-3(2001) [PubMed] [Europe PMC] [Abstract]
  7. "A conserved family of prolyl-4-hydroxylases that modify HIF."Science 294:1337-1340(2001) [PubMed] [Europe PMC] [Abstract]
  8. "Overexpression of PH-4, a novel putative proline 4-hydroxylase, modulates activity of hypoxia-inducible transcription factors."Biochem. Biophys. Res. Commun. 296:343-349(2002) [PubMed] [Europe PMC] [Abstract]
  9. "Sequence determinants in hypoxia-inducible factor-1alpha for hydroxylation by the prolyl hydroxylases PHD1, PHD2, and PHD3."J. Biol. Chem. 277:39792-39800(2002) [PubMed] [Europe PMC] [Abstract]
  10. "Differential regulation of HIF-1alpha prolyl-4-hydroxylase genes by hypoxia in human cardiovascular cells."Biochem. Biophys. Res. Commun. 303:947-953(2003) [PubMed] [Europe PMC] [Abstract]
  11. "Intracellular localisation of human HIF-1 alpha hydroxylases: implications for oxygen sensing."J. Cell Sci. 116:1319-1326(2003) [PubMed] [Europe PMC] [Abstract]
  12. "Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor."J. Biol. Chem. 279:38458-38465(2004) [PubMed] [Europe PMC] [Abstract]
  13. "Neuronal apoptosis linked to EglN3 prolyl hydroxylase and familial pheochromocytoma genes: developmental culling and cancer."Cancer Cell 8:155-167(2005) [PubMed] [Europe PMC] [Abstract]
  14. "Oxygen-regulated beta(2)-adrenergic receptor hydroxylation by EGLN3 and ubiquitylation by pVHL."Sci. Signal. 2:RA33-RA33(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Prolyl hydroxylase 3 interacts with Bcl-2 to regulate doxorubicin-induced apoptosis in H9c2 cells."Biochem. Biophys. Res. Commun. 401:231-237(2010) [PubMed] [Europe PMC] [Abstract]
  16. "PHD3 regulates differentiation, tumour growth and angiogenesis in pancreatic cancer."Br. J. Cancer 103:1571-1579(2010) [PubMed] [Europe PMC] [Abstract]
  17. "Prolyl hydroxylase domain protein 3 targets Pax2 for destruction."Biochem. Biophys. Res. Commun. 409:315-320(2011) [PubMed] [Europe PMC] [Abstract]
  18. "Biochemical characterization of human HIF hydroxylases using HIF protein substrates that contain all three hydroxylation sites."Biochem. J. 436:363-369(2011) [PubMed] [Europe PMC] [Abstract]
  19. "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1."Cell 145:732-744(2011) [PubMed] [Europe PMC] [Abstract]
  20. "The oxygen sensor PHD3 limits glycolysis under hypoxia via direct binding to pyruvate kinase."Cell Res. 21:983-986(2011) [PubMed] [Europe PMC] [Abstract]
  21. "Prolyl hydroxylase 3 (PHD3) is essential for hypoxic regulation of neutrophilic inflammation in humans and mice."J. Clin. Invest. 121:1053-1063(2011) [PubMed] [Europe PMC] [Abstract]
  22. "Effects of polynitrogen compounds on the activity of recombinant human HIF-1alpha prolyl hydroxylase 3 in E. coli."J. Inorg. Biochem. 105:391-399(2011) [PubMed] [Europe PMC] [Abstract]
  23. "PHD3-dependent hydroxylation of HCLK2 promotes the DNA damage response."J. Clin. Invest. 122:2827-2836(2012) [PubMed] [Europe PMC] [Abstract]
  24. "The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity."Nat. Cell Biol. 14:201-208(2012) [PubMed] [Europe PMC] [Abstract]