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DiGeorge Syndrome Critical Region Gene 8 (DGCR8)

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Gy1; DGCRK6; C22orf12; Microprocessor complex subunit DGCR8

DiGeorge Syndrome Critical Region Gene 8 (DGCR8)
CCC L429
HGNC 2847
MGI 2151114
PubMed 22796965
RGD 1306365
UniProt Q8WYQ5
Wiki DGCR8
DGCR8 interacted directly with Drosha, and the 2 proteins were present within a complex of about 650 kD. Both DGCR8 and Drosha could homodimerize in the absence of single-stranded RNA (ssRNA) in addition to interacting with each other. Depletion of DGCR8 from HeLa cells with small interfering RNA led to accumulation of pri-miRNA and loss of pre-miRNA and mature miRNA.
The deduced 773-amino acid protein contains a WW motif in its N-terminal half and 2 double-stranded RNA-binding motifs in its C-terminal half. In situ hybridization of mouse embryos revealed Dgcr8 expression in neuroepithelium of primary brain, limb bud, vessels, thymus, and palate during various developmental stages.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant DiGeorge Syndrome Critical Region Gene 8 (DGCR8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to DiGeorge Syndrome Critical Region Gene 8 (DGCR8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to DiGeorge Syndrome Critical Region Gene 8 (DGCR8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for DiGeorge Syndrome Critical Region Gene 8 (DGCR8) CLIA Kit Customized Service Offer
n/a ELISA Kit for DiGeorge Syndrome Critical Region Gene 8 (DGCR8) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant DiGeorge Syndrome Critical Region Gene 8 (DGCR8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to DiGeorge Syndrome Critical Region Gene 8 (DGCR8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to DiGeorge Syndrome Critical Region Gene 8 (DGCR8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for DiGeorge Syndrome Critical Region Gene 8 (DGCR8) CLIA Kit Customized Service Offer
n/a ELISA Kit for DiGeorge Syndrome Critical Region Gene 8 (DGCR8) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant DiGeorge Syndrome Critical Region Gene 8 (DGCR8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to DiGeorge Syndrome Critical Region Gene 8 (DGCR8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to DiGeorge Syndrome Critical Region Gene 8 (DGCR8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for DiGeorge Syndrome Critical Region Gene 8 (DGCR8) CLIA Kit Customized Service Offer
n/a ELISA Kit for DiGeorge Syndrome Critical Region Gene 8 (DGCR8) ELISA Kit Customized Service Offer
  1. "Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region."Biochem. Biophys. Res. Commun. 304:184-190(2003) [PubMed] [Europe PMC] [Abstract]
  2. "A genome annotation-driven approach to cloning the human ORFeome."Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence of human chromosome 22." Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  7. "Large-scale cDNA transfection screening for genes related to cancer development and progression." Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
  8. "The human DiGeorge syndrome critical region gene 8 and its D. melanogaster homolog are required for miRNA biogenesis."Curr. Biol. 14:2162-2167(2004) [PubMed] [Europe PMC] [Abstract]
  9. "The Drosha-DGCR8 complex in primary microRNA processing."Genes Dev. 18:3016-3027(2004) [PubMed] [Europe PMC] [Abstract]
  10. "The microprocessor complex mediates the genesis of microRNAs."Nature 432:235-240(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex."Cell 125:887-901(2006) [PubMed] [Europe PMC] [Abstract]
  12. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Formation of GW bodies is a consequence of microRNA genesis."EMBO Rep. 7:904-910(2006) [PubMed] [Europe PMC] [Abstract]
  14. "Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing."Nucleic Acids Res. 34:4622-4629(2006) [PubMed] [Europe PMC] [Abstract]
  15. "Nucleolar localization of DGCR8 and identification of eleven DGCR8-associated proteins."Exp. Cell Res. 313:4196-4207(2007) [PubMed] [Europe PMC] [Abstract]
  16. "Heme is involved in microRNA processing."Nat. Struct. Mol. Biol. 14:23-29(2007) [PubMed] [Europe PMC] [Abstract]
  17. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  18. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  19. "Posttranscriptional crossregulation between Drosha and DGCR8."Cell 136:75-84(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  22. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  23. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  24. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  25. "N6-methyladenosine marks primary microRNAs for processing."Nature 519:482-485(2015) [PubMed] [Europe PMC] [Abstract]
  26. "Crystal structure of human DGCR8 core."Nat. Struct. Mol. Biol. 14:847-853(2007) [PubMed] [Europe PMC] [Abstract]
  27. "Solution structure of DSRM domain in DGCR8 protein."Submitted (NOV-2005) to the PDB data bank