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CCCTC Binding Factor (CTCF)

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11 Zinc Finger Transcriptional Repressor; CTCFL paralog; Transcriptional repressor CTCF

CCCTC Binding Factor (CTCF)
CCC L385
HGNC 13723
MGI 109447
PubMed 24174344
RGD 621344
UniProt P49711
Wiki CTCF
CTCF is a major protein involved in insulator activity. The protein CTCF plays heavily in the role of repressing the insulin-like growth factor 2 gene, by binding to the H-19 imprinting control region (ICR) along with Differentially-methylated Region-1 (DMR1) and MAR3.Binding of targeting sequence elements by CTCF can block the interaction between enhancers and promoters, therefore limiting the activity of enhancers to certain functional domains. Besides acting as enhancer blocking, CTCF can also act as a chromatin barrier by preventing the spread of heterochromatin structures.In 2007, two independent studies (Xie et al. from MIT and Kim et al. from UCSD) revealed that the human genome contains nearly 15,000 CTCF insulator sites, suggesting a widespread role of CTCF in gene regulation.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant CCCTC Binding Factor (CTCF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to CCCTC Binding Factor (CTCF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to CCCTC Binding Factor (CTCF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for CCCTC Binding Factor (CTCF) CLIA Kit Customized Service Offer
n/a ELISA Kit for CCCTC Binding Factor (CTCF) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant CCCTC Binding Factor (CTCF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to CCCTC Binding Factor (CTCF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to CCCTC Binding Factor (CTCF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for CCCTC Binding Factor (CTCF) CLIA Kit Customized Service Offer
n/a ELISA Kit for CCCTC Binding Factor (CTCF) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant CCCTC Binding Factor (CTCF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to CCCTC Binding Factor (CTCF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to CCCTC Binding Factor (CTCF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for CCCTC Binding Factor (CTCF) CLIA Kit Customized Service Offer
n/a ELISA Kit for CCCTC Binding Factor (CTCF) ELISA Kit Customized Service Offer
  1. "An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes."Mol. Cell. Biol. 16:2802-2813(1996) [PubMed] [Europe PMC] [Abstract]
  2. "A widely expressed transcription factor with multiple DNA sequence specificity, CTCF, is localized at chromosome segment 16q22.1 within one of the smallest regions of overlap for common deletions in breast and prostate cancers."Genes Chromosomes Cancer 22:26-36(1998) [PubMed] [Europe PMC] [Abstract]
  3. "Tumor-associated zinc finger mutations in the CTCF transcription factor selectively alter its DNA-binding specificity." Cancer Res. 62:48-52(2002) [PubMed] [Europe PMC] [Abstract]
  4. "The sequence and analysis of duplication-rich human chromosome 16." Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The zinc finger protein CTCF binds to the APBbeta domain of the amyloid beta-protein precursor promoter. Evidence for a role in transcriptional activation."J. Biol. Chem. 272:33353-33359(1997) [PubMed] [Europe PMC] [Abstract]
  7. "The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer."Semin. Cancer Biol. 12:399-414(2002) [PubMed] [Europe PMC] [Abstract]
  8. "CTCF, a candidate trans-acting factor for X-inactivation choice."Science 295:345-347(2002) [PubMed] [Europe PMC] [Abstract]
  9. "CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin."EMBO J. 24:3291-3300(2005) [PubMed] [Europe PMC] [Abstract]
  10. "Targeting of CTCF to the nucleolus inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanism."J. Cell Sci. 119:1746-1759(2006) [PubMed] [Europe PMC] [Abstract]
  11. "CTCF-dependent chromatin insulator is linked to epigenetic remodeling."Mol. Cell 23:733-742(2006) [PubMed] [Europe PMC] [Abstract]
  12. "CTCF binding at the H19 imprinting control region mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to Igf2."Proc. Natl. Acad. Sci. U.S.A. 103:10684-10689(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Critical DNA binding interactions of the insulator protein CTCF: a small number of zinc fingers mediate strong binding, and a single finger-DNA interaction controls binding at imprinted loci."J. Biol. Chem. 282:33336-33345(2007) [PubMed] [Europe PMC] [Abstract]
  14. "DNA methyltransferase 1 and 3B activate BAG-1 expression via recruitment of CTCFL/BORIS and modulation of promoter histone methylation."Cancer Res. 68:2726-2735(2008) [PubMed] [Europe PMC] [Abstract]
  15. "The insulator factor CTCF controls MHC class II gene expression and is required for the formation of long-distance chromatin interactions."J. Exp. Med. 205:785-798(2008) [PubMed] [Europe PMC] [Abstract]
  16. "The insulator binding protein CTCF positions 20 nucleosomes around its binding sites across the human genome."PLoS Genet. 4:E1000138-E1000138(2008) [PubMed] [Europe PMC] [Abstract]
  17. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  18. "CTCF physically links cohesin to chromatin."Proc. Natl. Acad. Sci. U.S.A. 105:8309-8314(2008) [PubMed] [Europe PMC] [Abstract]
  19. "Architectural roles of multiple chromatin insulators at the human apolipoprotein gene cluster."EMBO J. 28:1234-1245(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  23. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  24. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  25. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  26. "Solution structure of zinc finger domains of transcriptional repressor CTCF protein."Submitted (NOV-2005) to the PDB data bank
  27. "De novo mutations in the genome organizer CTCF cause intellectual disability."Am. J. Hum. Genet. 93:124-131(2013) [PubMed] [Europe PMC] [Abstract]