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RAD18 Homolog (RAD18)

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RNF73; E3 ubiquitin-protein ligase RAD18; Postreplication repair protein RAD18; RING finger protein 73

RAD18 Homolog (RAD18)
CCC K280
HGNC 18278
MGI 1890476
PubMed 21884979
RGD 1306993
UniProt Q9NS91
Wiki RAD18
RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. Human RAD18 encodes a deduced 495-amino acid protein that shares 20% sequence identity and 42% similarity with yeast Rad18. Human RAD18 localizes in the nucleus. In vivo the human RAD18 protein binds to the human homologs of the yeast Rad6 protein through a conserved ring finger motif.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RAD18 Homolog (RAD18) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RAD18 Homolog (RAD18) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RAD18 Homolog (RAD18) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RAD18 Homolog (RAD18) CLIA Kit Customized Service Offer
n/a ELISA Kit for RAD18 Homolog (RAD18) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RAD18 Homolog (RAD18) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RAD18 Homolog (RAD18) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RAD18 Homolog (RAD18) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RAD18 Homolog (RAD18) CLIA Kit Customized Service Offer
n/a ELISA Kit for RAD18 Homolog (RAD18) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RAD18 Homolog (RAD18) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RAD18 Homolog (RAD18) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RAD18 Homolog (RAD18) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RAD18 Homolog (RAD18) CLIA Kit Customized Service Offer
n/a ELISA Kit for RAD18 Homolog (RAD18) ELISA Kit Customized Service Offer
  1. "Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens."Proc. Natl. Acad. Sci. U.S.A. 97:7927-7932(2000) [PubMed] [Europe PMC] [Abstract]
  2. "The human RAD18 gene product interacts with HHR6A and HHR6B."Nucleic Acids Res. 28:2847-2854(2000) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence, annotation and analysis of human chromosome 3." Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  7. "Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination."J. Cell Biol. 175:703-708(2006) [PubMed] [Europe PMC] [Abstract]
  8. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  9. "Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen."Proc. Natl. Acad. Sci. U.S.A. 103:18107-18112(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  11. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination."Proc. Natl. Acad. Sci. U.S.A. 105:3768-3773(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks."Proc. Natl. Acad. Sci. U.S.A. 105:12411-12416(2008) [PubMed] [Europe PMC] [Abstract]
  14. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  18. "A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling."Science 332:1313-1317(2011) [PubMed] [Europe PMC] [Abstract]
  19. "Tandem protein interaction modules organize the ubiquitin-dependent response to DNA double-strand breaks."Mol. Cell 47:383-395(2012) [PubMed] [Europe PMC] [Abstract]
  20. "Spartan/C1orf124, a reader of PCNA ubiquitylation and a regulator of UV-induced DNA damage response."Mol. Cell 46:625-635(2012) [PubMed] [Europe PMC] [Abstract]
  21. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  22. "Symmetry and asymmetry of the RING-RING dimer of Rad18."J. Mol. Biol. 410:424-435(2011) [PubMed] [Europe PMC] [Abstract]