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RNA Polymerase II, DNA Directed Polypeptide A (POLR2A)

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POLR2; POLRA; RPB1; RPBh1; RPO2; RPOL2; RpIILS; hRPB220; hsRPB1; DNA-directed RNA polymerase II subunit RPB1

RNA Polymerase II, DNA Directed Polypeptide A (POLR2A)
CCC K090
HGNC 9187
MGI 98086
PubMed 23638030
RGD 1587326
UniProt P24928
Wiki POLR2A
POLR2A encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase.
In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.The extent of this transcript is supported by transcript alignments.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) CLIA Kit Customized Service Offer
n/a ELISA Kit for RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) CLIA Kit Customized Service Offer
n/a ELISA Kit for RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) CLIA Kit Customized Service Offer
n/a ELISA Kit for RNA Polymerase II, DNA Directed Polypeptide A (POLR2A) ELISA Kit Customized Service Offer
  1. "Complete sequence of the human RNA polymerase II largest subunit."Nucleic Acids Res. 20:910-910(1992) [PubMed] [Europe PMC] [Abstract]
  2. "The human gene encoding the largest subunit of RNA polymerase II."Gene 159:285-286(1995) [PubMed] [Europe PMC] [Abstract]
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes."J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract]
  6. "SAF-B couples transcription and pre-mRNA splicing to SAR/MAR elements."Nucleic Acids Res. 26:3542-3549(1998) [PubMed] [Europe PMC] [Abstract]
  7. "A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription."EMBO J. 18:3688-3701(1999) [PubMed] [Europe PMC] [Abstract]
  8. "Tat-SF1 protein associates with RAP30 and human SPT5 proteins."Mol. Cell. Biol. 19:5960-5968(1999) [PubMed] [Europe PMC] [Abstract]
  9. "The structure of an FF domain from human HYPA/FBP11."J. Mol. Biol. 323:411-416(2002) [PubMed] [Europe PMC] [Abstract]
  10. "Hyperphosphorylated C-terminal repeat domain-associating proteins in the nuclear proteome link transcription to DNA/chromatin modification and RNA processing."Mol. Cell. Proteomics 1:598-610(2002) [PubMed] [Europe PMC] [Abstract]
  11. "Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300."Oncogene 22:151-156(2003) [PubMed] [Europe PMC] [Abstract]
  12. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
  13. "Cyclin L2, a novel RNA polymerase II-associated cyclin, is involved in pre-mRNA splicing and induces apoptosis of human hepatocellular carcinoma cells."J. Biol. Chem. 279:11639-11648(2004) [PubMed] [Europe PMC] [Abstract]
  14. "Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus."Mol. Cell 13:587-597(2004) [PubMed] [Europe PMC] [Abstract]
  15. "Expression of the C-terminal domain of novel human SR-A1 protein: interaction with the CTD domain of RNA polymerase II."Biochem. Biophys. Res. Commun. 334:61-68(2005) [PubMed] [Europe PMC] [Abstract]
  16. "Identification and characterization of a novel human histone H3 lysine 36 specific methyltransferase."J. Biol. Chem. 280:35261-35271(2005) [PubMed] [Europe PMC] [Abstract]
  17. "Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1."Proc. Natl. Acad. Sci. U.S.A. 102:17636-17641(2005) [PubMed] [Europe PMC] [Abstract]
  18. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  19. "The human homologue of the RNA polymerase II-associated factor 1 (hPaf1), localized on the 19q13 amplicon, is associated with tumorigenesis."Oncogene 25:3247-3257(2006) [PubMed] [Europe PMC] [Abstract]
  20. "The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export."Genes Dev. 21:160-174(2007) [PubMed] [Europe PMC] [Abstract]
  21. "The human interferon-regulated ISG95 protein interacts with RNA polymerase II and shows methyltransferase activity."Biochem. Biophys. Res. Commun. 372:719-724(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the carboxyl-terminal domain of RNA polymerase II."J. Biol. Chem. 283:22659-22669(2008) [PubMed] [Europe PMC] [Abstract]
  23. "Transcription of hepatitis delta virus RNA by RNA polymerase II."J. Virol. 82:1118-1127(2008) [PubMed] [Europe PMC] [Abstract]
  24. "Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes."Mol. Cell. Biol. 28:609-618(2008) [PubMed] [Europe PMC] [Abstract]
  25. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  26. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  27. "MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity."J. Biol. Chem. 284:5165-5174(2009) [PubMed] [Europe PMC] [Abstract]
  28. "TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II."Mol. Cell 34:387-393(2009) [PubMed] [Europe PMC] [Abstract]
  29. "TFIIH-associated Cdk7 kinase functions in phosphorylation of C-terminal domain Ser7 residues, promoter-proximal pausing, and termination by RNA polymerase II."Mol. Cell. Biol. 29:5455-5464(2009) [PubMed] [Europe PMC] [Abstract]
  30. "Binding to DNA of the RNA-polymerase II C-terminal domain allows discrimination between Cdk7 and Cdk9 phosphorylation."Nucleic Acids Res. 37:1260-1268(2009) [PubMed] [Europe PMC] [Abstract]
  31. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  32. "RecQL5 promotes genome stabilization through two parallel mechanisms--interacting with RNA polymerase II and acting as a helicase."Mol. Cell. Biol. 30:2460-2472(2010) [PubMed] [Europe PMC] [Abstract]
  33. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  34. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  35. "The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex."Genes Dev. 25:972-983(2011) [PubMed] [Europe PMC] [Abstract]
  36. "Transcription factor IIS cooperates with the E3 ligase UBR5 to ubiquitinate the CDK9 subunit of the positive transcription elongation factor B."J. Biol. Chem. 286:5012-5022(2011) [PubMed] [Europe PMC] [Abstract]
  37. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  38. "The C-terminal domain of RNA polymerase II is modified by site-specific methylation."Science 332:99-103(2011) [PubMed] [Europe PMC] [Abstract]
  39. "Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes."Mol. Cell 45:111-122(2012) [PubMed] [Europe PMC] [Abstract]
  40. "Mutations in UVSSA cause UV-sensitive syndrome and impair RNA polymerase IIo processing in transcription-coupled nucleotide-excision repair." Nat. Genet. 44:586-592(2012) [PubMed] [Europe PMC] [Abstract]
  41. "Herpes simplex virus 1 ICP22 inhibits the transcription of viral gene promoters by binding to and blocking the recruitment of P-TEFb."PLoS ONE 7:E45749-E45749(2012) [PubMed] [Europe PMC] [Abstract]
  42. "Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor apraxia 2, lies at the interface of transcription and the DNA damage response."Mol. Cell. Biol. 33:406-417(2013) [PubMed] [Europe PMC] [Abstract]
  43. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  44. "Phosphorylation-dependent PIH1D1 interactions define substrate specificity of the R2TP cochaperone complex."Cell Rep. 7:19-26(2014) [PubMed] [Europe PMC] [Abstract]
  45. "Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1."Mol. Cell 24:759-770(2006) [PubMed] [Europe PMC] [Abstract]
  46. "Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5."Nat. Struct. Mol. Biol. 20:892-899(2013) [PubMed] [Europe PMC] [Abstract]