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Budding Uninhibited By Benzimidazoles 1 (BUB1)

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BUB1A; BUB1L; hBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1

Budding Uninhibited By Benzimidazoles 1 (BUB1)
CCC K067
HGNC 1148
MGI 1100510
PubMed 23747338
RGD 1311707
UniProt O43683
Wiki BUB1
Bub1 is a serine/threonine protein kinase first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of Bub1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence.
Bub1p comprises a conserved N-terminal region, a central non-conserved region and a C-terminal serine/threonine kinase domain. The N-terminal region mediates binding of Hs-BUB1 to the mitotic kinetochore protein blinkin.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPK067Hu01 Recombinant Budding Uninhibited By Benzimidazoles 1 (BUB1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Budding Uninhibited By Benzimidazoles 1 (BUB1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Budding Uninhibited By Benzimidazoles 1 (BUB1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Budding Uninhibited By Benzimidazoles 1 (BUB1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Budding Uninhibited By Benzimidazoles 1 (BUB1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Budding Uninhibited By Benzimidazoles 1 (BUB1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Budding Uninhibited By Benzimidazoles 1 (BUB1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Budding Uninhibited By Benzimidazoles 1 (BUB1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Budding Uninhibited By Benzimidazoles 1 (BUB1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Budding Uninhibited By Benzimidazoles 1 (BUB1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Budding Uninhibited By Benzimidazoles 1 (BUB1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Budding Uninhibited By Benzimidazoles 1 (BUB1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Budding Uninhibited By Benzimidazoles 1 (BUB1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Budding Uninhibited By Benzimidazoles 1 (BUB1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Budding Uninhibited By Benzimidazoles 1 (BUB1) ELISA Kit Customized Service Offer
  1. "Mutations of mitotic checkpoint genes in human cancers."Nature 392:300-303(1998) [PubMed] [Europe PMC] [Abstract]
  2. "Human Bub1: a putative spindle checkpoint kinase closely linked to cell proliferation."Cell Growth Differ. 9:877-885(1998) [PubMed] [Europe PMC] [Abstract]
  3. "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase."J. Cell Biol. 142:1-11(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Phosphorylation of human MAD1 by the BUB1 kinase in vitro."Biochem. Biophys. Res. Commun. 257:589-595(1999) [PubMed] [Europe PMC] [Abstract]
  5. "Characterization of MAD2B and other mitotic spindle checkpoint genes."Genomics 58:181-187(1999) [PubMed] [Europe PMC] [Abstract]
  6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Mammalian BUB1 protein kinases: map positions and in vivo expression."Genomics 46:379-388(1997) [PubMed] [Europe PMC] [Abstract]
  9. "Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression."J. Cell Sci. 117:1577-1589(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint."Mol. Cell 16:387-397(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Human Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting Shugoshin localization."Curr. Biol. 15:353-359(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1."Mol. Biol. Cell 17:3705-3716(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1."Dev. Cell 13:663-676(2007) [PubMed] [Europe PMC] [Abstract]
  14. "KEN-box-dependent degradation of the Bub1 spindle checkpoint kinase by the anaphase-promoting complex/cyclosome."J. Biol. Chem. 282:3672-3679(2007) [PubMed] [Europe PMC] [Abstract]
  15. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  16. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  17. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  18. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding."J. Virol. 83:117-127(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Bub1 regulates chromosome segregation in a kinetochore-independent manner."J. Cell Biol. 185:841-858(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  24. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  25. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  26. "A double missense variation of the BUB1 gene and a defective mitotic spindle checkpoint in the pancreatic cancer cell line Hs766T."Hum. Mutat. 21:445-445(2003) [PubMed] [Europe PMC] [Abstract]
  27. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]