≡

Aldo Keto Reductase Family 1, Member D1 (AKR1D1)

[Edit]

SRD5B1; Delta 4-3-Ketosteroid-5-Beta-Reductase; 3-oxo-5-beta-steroid 4-dehydrogenase; Delta(4)-3-oxosteroid 5-beta-reductase; Aldo-keto reductase family 1 member D1

Aldo Keto Reductase Family 1, Member D1 (AKR1D1)
CCC J654
HGNC 388
MGI 2384785
PubMed 23679950
RGD 620752
UniProt P51857
Human delta(4)-3-oxosteroid 5-beta-reductase (steroid 5-beta-reductase) catalyzes 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure.
The full-length cDNA encodes a peptide of 326 amino acids with a relative mass of 37,380 Da. Northern blot analysis of human liver RNA identified a 2.7-kb transcript. The authors inserted the 5-beta-reductase cDNA into an expression vector and transfected it into COS cells. The recombinant protein was detected by immunoblot analysis using a monoclonal antibody against rat 5-beta-reductase. Recombinant 5-beta-reductase showed 5-beta-reductase activity against several substrates, as detected by high-performance liquid chromatography.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Aldo Keto Reductase Family 1, Member D1 (AKR1D1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Aldo Keto Reductase Family 1, Member D1 (AKR1D1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Aldo Keto Reductase Family 1, Member D1 (AKR1D1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Aldo Keto Reductase Family 1, Member D1 (AKR1D1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Aldo Keto Reductase Family 1, Member D1 (AKR1D1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Aldo Keto Reductase Family 1, Member D1 (AKR1D1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Aldo Keto Reductase Family 1, Member D1 (AKR1D1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Aldo Keto Reductase Family 1, Member D1 (AKR1D1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Aldo Keto Reductase Family 1, Member D1 (AKR1D1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Aldo Keto Reductase Family 1, Member D1 (AKR1D1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Aldo Keto Reductase Family 1, Member D1 (AKR1D1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Aldo Keto Reductase Family 1, Member D1 (AKR1D1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Aldo Keto Reductase Family 1, Member D1 (AKR1D1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Aldo Keto Reductase Family 1, Member D1 (AKR1D1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Aldo Keto Reductase Family 1, Member D1 (AKR1D1) ELISA Kit Customized Service Offer
  1. "Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme."Eur. J. Biochem. 219:357-363(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Genomic organization of a human 5beta-reductase and its pseudogene and substrate selectivity of the expressed enzyme."Biochim. Biophys. Acta 1517:228-235(2001) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence of human chromosome 7." Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
  5. "Human chromosome 7: DNA sequence and biology." Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  9. "The crystal structure of human Delta4-3-ketosteroid 5beta-reductase defines the functional role of the residues of the catalytic tetrad in the steroid double bond reduction mechanism."Biochemistry 47:8261-8270(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Crystal structures of human Delta4-3-ketosteroid 5beta-reductase (AKR1D1) reveal the presence of an alternative binding site responsible for substrate inhibition."Biochemistry 47:13537-13546(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Crystal structure of human liver delta(4)-3-ketosteroid 5beta-reductase (AKR1D1) and implications for substrate binding and catalysis."J. Biol. Chem. 283:16830-16839(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Inhibition of human steroid 5beta-reductase (AKR1D1) by finasteride and structure of the enzyme-inhibitor complex."J. Biol. Chem. 284:19786-19790(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Structure and catalytic mechanism of human steroid 5beta-reductase (AKR1D1)."Mol. Cell. Endocrinol. 301:191-198(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Conversion of human steroid 5beta-reductase (AKR1D1) into 3beta-hydroxysteroid dehydrogenase by single point mutation E120H: example of perfect enzyme engineering."J. Biol. Chem. 287:16609-16622(2012) [PubMed] [Europe PMC] [Abstract]
  15. "Mutations in SRD5B1 (AKR1D1), the gene encoding delta(4)-3-oxosteroid 5beta-reductase, in hepatitis and liver failure in infancy."Gut 52:1494-1499(2003) [PubMed] [Europe PMC] [Abstract]
  16. "SRD5B1 (AKR1D1) gene analysis in delta(4)-3-oxosteroid 5beta-reductase deficiency: evidence for primary genetic defect."J. Hepatol. 40:716-718(2004) [PubMed] [Europe PMC] [Abstract]