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ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1)

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P200; ARFGEP1; BIG1; Brefeldin A-inhibited guanine nucleotide-exchange protein 1; p200 ARF guanine nucleotide exchange factor

ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1)
CCC J610
HGNC 15772
MGI 2442988
PubMed 21871176
RGD 1560793
UniProt Q9Y6D6
Wiki ARFGEF1
ARFGEF1 is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for the guanine-nucleotide exchange activity and also the brefeldin A inhibition.
BIG1 and MYO9B bound with a 1-to-1 stoichiometry, and mutation analysis showed that BIG1 bound specifically to the zinc finger/GAP domain of MYO9B. Binding of BIG1 to MYO9B interfered with binding of RhoA to MYO9B and inhibited the Rho-GAP activity of MYO9B in a dose-dependent manner. Likewise, RhoA inhibited binding of BIG1 to MYO9B. Binding of MYO9B to BIG1 did not alter the ARF-GEF activity of BIG1.The full-length BIG1 cDNA encodes a protein of 1,849 amino acids containing a Sec7 domain characteristic of ARF guanine nucleotide-exchange proteins.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1 (ARFGEF1) ELISA Kit Customized Service Offer
  1. "Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors."J. Biol. Chem. 274:12308-12315(1999) [PubMed] [Europe PMC] [Abstract]
  2. "p200 ARF-GEP1: a Golgi-localized guanine nucleotide exchange protein whose Sec7 domain is targeted by the drug brefeldin A."Proc. Natl. Acad. Sci. U.S.A. 96:7968-7973(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  5. "Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex."Proc. Natl. Acad. Sci. U.S.A. 97:2567-2572(2000) [PubMed] [Europe PMC] [Abstract]
  6. "Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)."Proc. Natl. Acad. Sci. U.S.A. 100:1627-1632(2003) [PubMed] [Europe PMC] [Abstract]
  7. "Interaction of FK506-binding protein 13 with brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1): effects of FK506."Proc. Natl. Acad. Sci. U.S.A. 100:2322-2327(2003) [PubMed] [Europe PMC] [Abstract]
  8. "Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors."Proc. Natl. Acad. Sci. U.S.A. 101:2752-2757(2004) [PubMed] [Europe PMC] [Abstract]
  9. "BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity."J. Biol. Chem. 280:10128-10134(2005) [PubMed] [Europe PMC] [Abstract]
  10. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  11. "Effect of protein kinase A on accumulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) in HepG2 cell nuclei."Proc. Natl. Acad. Sci. U.S.A. 103:2683-2688(2006) [PubMed] [Europe PMC] [Abstract]
  12. "Interactions between conserved domains within homodimers in the BIG1, BIG2, and GBF1 Arf guanine nucleotide exchange factors."J. Biol. Chem. 282:28834-28842(2007) [PubMed] [Europe PMC] [Abstract]
  13. "BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein, is required for correct glycosylation and function of integrin beta1."Proc. Natl. Acad. Sci. U.S.A. 104:1230-1235(2007) [PubMed] [Europe PMC] [Abstract]
  14. "Regulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) and BIG2 activity via PKA and protein phosphatase 1gamma."Proc. Natl. Acad. Sci. U.S.A. 104:3201-3206(2007) [PubMed] [Europe PMC] [Abstract]
  15. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  16. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Association of guanine nucleotide-exchange protein BIG1 in HepG2 cell nuclei with nucleolin, U3 snoRNA, and fibrillarin."Proc. Natl. Acad. Sci. U.S.A. 105:3357-3361(2008) [PubMed] [Europe PMC] [Abstract]
  18. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  19. "A role of histone H3 lysine 4 methyltransferase components in endosomal trafficking."J. Cell Biol. 186:343-353(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity."Proc. Natl. Acad. Sci. U.S.A. 106:6158-6163(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  23. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  24. "Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing."Proc. Natl. Acad. Sci. U.S.A. 108:19228-19233(2011) [PubMed] [Europe PMC] [Abstract]
  25. "Specific functions of BIG1 and BIG2 in endomembrane organization."PLoS ONE 5:E9898-E9898(2010) [PubMed] [Europe PMC] [Abstract]
  26. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  27. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  28. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]