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ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2)

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BIG2; ARFGEP2; Brefeldin A-Inhibited; Brefeldin A-inhibited guanine nucleotide-exchange protein 2

ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2)
CCC J609
HGNC 15853
MGI 2139354
PubMed 14647276
RGD 631430
UniProt Q9Y6D5
Wiki ARFGEF2
ARFGEF2 encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.BIG2 encodes a protein of 1,785 amino acids containing a Sec7 domain characteristic of other ADP ribosylation factor (ARF) guanine nucleotide exchange proteins. BIG2 shares 74% overall amino acid identity with BIG1 and 90% identity within the Sec7 domain. BIG2 protein is required for vesicle and membrane trafficking from the trans-Golgi network. Inhibition of BIG2 by BFA, or by a dominant-negative ARFGEF2 cDNA, decreased cell proliferation in vitro, suggesting a cell-autonomous regulation of neural expansion.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) CLIA Kit Customized Service Offer
n/a ELISA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) CLIA Kit Customized Service Offer
n/a ELISA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) CLIA Kit Customized Service Offer
n/a ELISA Kit for ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2 (ARFGEF2) ELISA Kit Customized Service Offer
  1. "Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors."J. Biol. Chem. 274:12308-12315(1999) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and comparative analysis of human chromosome 20." Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
  3. "Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex."Proc. Natl. Acad. Sci. U.S.A. 97:2567-2572(2000) [PubMed] [Europe PMC] [Abstract]
  4. "Dominant-negative mutant of BIG2, an ARF-guanine nucleotide exchange factor, specifically affects membrane trafficking from the trans-Golgi network through inhibiting membrane association of AP-1 and GGA coat proteins."Biochem. Biophys. Res. Commun. 294:254-260(2002) [PubMed] [Europe PMC] [Abstract]
  5. "Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)."Proc. Natl. Acad. Sci. U.S.A. 100:1627-1632(2003) [PubMed] [Europe PMC] [Abstract]
  6. "BIG2, a guanine nucleotide exchange factor for ADP-ribosylation factors: its localization to recycling endosomes and implication in the endosome integrity."Mol. Biol. Cell 15:5283-5294(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70."Proc. Natl. Acad. Sci. U.S.A. 102:2784-2789(2005) [PubMed] [Europe PMC] [Abstract]
  8. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  9. "AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors."Genes Cells 11:949-959(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake."Proc. Natl. Acad. Sci. U.S.A. 103:2635-2640(2006) [PubMed] [Europe PMC] [Abstract]
  11. "The brefeldin A-inhibited guanine nucleotide-exchange protein, BIG2, regulates the constitutive release of TNFR1 exosome-like vesicles."J. Biol. Chem. 282:9591-9599(2007) [PubMed] [Europe PMC] [Abstract]
  12. "Interactions between conserved domains within homodimers in the BIG1, BIG2, and GBF1 Arf guanine nucleotide exchange factors."J. Biol. Chem. 282:28834-28842(2007) [PubMed] [Europe PMC] [Abstract]
  13. "Regulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) and BIG2 activity via PKA and protein phosphatase 1gamma."Proc. Natl. Acad. Sci. U.S.A. 104:3201-3206(2007) [PubMed] [Europe PMC] [Abstract]
  14. "cAMP-dependent protein kinase A (PKA) signaling induces TNFR1 exosome-like vesicle release via anchoring of PKA regulatory subunit RIIbeta to BIG2."J. Biol. Chem. 283:25364-25371(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  16. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity."Proc. Natl. Acad. Sci. U.S.A. 106:6158-6163(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Specific functions of BIG1 and BIG2 in endomembrane organization."PLoS ONE 5:E9898-E9898(2010) [PubMed] [Europe PMC] [Abstract]
  22. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  23. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  24. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  25. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  26. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  27. "Mutations in ARFGEF2 implicate vesicle trafficking in neural progenitor proliferation and migration in the human cerebral cortex."Nat. Genet. 36:69-76(2004) [PubMed] [Europe PMC] [Abstract]
  28. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
  29. "Diagnostic exome sequencing in persons with severe intellectual disability."N. Engl. J. Med. 367:1921-1929(2012) [PubMed] [Europe PMC] [Abstract]