≡

Chromatin Licensing And DNA Replication Factor 1 (CDT1)

[Edit]

DUP; RIS2; DNA replication factor Cdt1; Double parked homolog

Chromatin Licensing And DNA Replication Factor 1 (CDT1)
CCC J461
HGNC 24576
MGI 1914427
PubMed 22451935
RGD 1309211
UniProt Q9H211
Wiki CDT1
CDT1 is a key licensing factor which, along with the protein Cdc6, functions to license DNA by forming the pre-replicative complex (pre-RC). Its activity during the cell cycle is tightly regulated by its association with the protein geminin, which both inhibits Cdt1 activity during S phase in order to prevent re-replication of DNA and prevents it from ubiquitination and subsequent proteolysis.
human CDT1 is a 65-kD protein that contains 546 amino acids and coimmunoprecipitates with geminin. Inhibition of DNA replication by geminin in cell-free cDNA replication extracts could be reversed by the addition of excess CDT1. In the normal cell cycle, CDT1 is present only in G1 and S phases, whereas geminin is present in S and G2 phases.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Chromatin Licensing And DNA Replication Factor 1 (CDT1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Chromatin Licensing And DNA Replication Factor 1 (CDT1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Chromatin Licensing And DNA Replication Factor 1 (CDT1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Chromatin Licensing And DNA Replication Factor 1 (CDT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Chromatin Licensing And DNA Replication Factor 1 (CDT1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Chromatin Licensing And DNA Replication Factor 1 (CDT1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Chromatin Licensing And DNA Replication Factor 1 (CDT1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Chromatin Licensing And DNA Replication Factor 1 (CDT1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Chromatin Licensing And DNA Replication Factor 1 (CDT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Chromatin Licensing And DNA Replication Factor 1 (CDT1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Chromatin Licensing And DNA Replication Factor 1 (CDT1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Chromatin Licensing And DNA Replication Factor 1 (CDT1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Chromatin Licensing And DNA Replication Factor 1 (CDT1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Chromatin Licensing And DNA Replication Factor 1 (CDT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Chromatin Licensing And DNA Replication Factor 1 (CDT1) ELISA Kit Customized Service Offer
  1. "Inhibition of eukaryotic DNA replication by geminin binding to Cdt1."Science 290:2309-2312(2000) [PubMed] [Europe PMC] [Abstract]
  2. "The Cdt1 protein is required to license DNA for replication in fission yeast."Nature 404:625-628(2000) [PubMed] [Europe PMC] [Abstract]
  3. "The sequence and analysis of duplication-rich human chromosome 16." Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract], Cervix, Eye, Kidney, Liver and Uterus.
  5. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  7. "JNK1 phosphorylation of Cdt1 inhibits recruitment of HBO1 histone acetylase and blocks replication licensing in response to stress."Mol. Cell 44:62-71(2011) [PubMed] [Europe PMC] [Abstract]
  8. "Human geminin promotes pre-RC formation and DNA replication by stabilizing CDT1 in mitosis."EMBO J. 23:3122-3132(2004) [PubMed] [Europe PMC] [Abstract]
  9. "The regulated association of Cdt1 with minichromosome maintenance proteins and Cdc6 in mammalian cells."J. Biol. Chem. 279:9625-9633(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding."J. Biol. Chem. 279:19691-19697(2004) [PubMed] [Europe PMC] [Abstract]
  11. "L2DTL/CDT2 interacts with the CUL4/DDB1 complex and PCNA and regulates CDT1 proteolysis in response to DNA damage."Cell Cycle 5:1675-1680(2006) [PubMed] [Europe PMC] [Abstract]
  12. "DTL/CDT2 is essential for both CDT1 regulation and the early G2/M checkpoint."Genes Dev. 20:3117-3129(2006) [PubMed] [Europe PMC] [Abstract]
  13. "A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1."Mol. Cell 23:709-721(2006) [PubMed] [Europe PMC] [Abstract]
  14. "Idas, a novel phylogenetically conserved geminin-related protein, binds to geminin and is required for cell cycle progression."J. Biol. Chem. 286:23234-23246(2011) [PubMed] [Europe PMC] [Abstract]
  15. "Dynamic association of ORCA with prereplicative complex components regulates DNA replication initiation."Mol. Cell. Biol. 32:3107-3120(2012) [PubMed] [Europe PMC] [Abstract]
  16. "Mutations in the pre-replication complex cause Meier-Gorlin syndrome." Nat. Genet. 43:356-359(2011) [PubMed] [Europe PMC] [Abstract]
  17. "Mutations in origin recognition complex gene ORC4 cause Meier-Gorlin syndrome." Nat. Genet. 43:360-364(2011) [PubMed] [Europe PMC] [Abstract]