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Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR)

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RNF116; RNF196; RING finger protein 196; E3 ubiquitin-protein ligase CHFR

Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR)
CCC J373
HGNC 20455
MGI 2444898
PubMed 10935642
RGD 1306360
UniProt Q96EP1
Wiki CHFR
CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. Expression of CHFR was precisely correlated with both CpG methylation and deacetylation of histones H3 and H4 in the CpG-rich regulatory region. Cells with CHFR methylation had an intrinsically high mitotic index when treated with microtubule inhibitor. This means that cells in which CHFR was epigenetically inactivated constituted loss-of-function alleles for mitotic checkpoint control. Taken together, these findings shed light on the pathway by which the mitotic checkpoint is bypassed in cancer cells and suggest that inactivation of checkpoint genes is much more widespread than previously suspected.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) CLIA Kit Customized Service Offer
n/a ELISA Kit for Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) CLIA Kit Customized Service Offer
n/a ELISA Kit for Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) CLIA Kit Customized Service Offer
n/a ELISA Kit for Checkpoint With Forkhead And Ring Finger Domains Protein (CHFR) ELISA Kit Customized Service Offer
  1. "Chfr defines a mitotic stress checkpoint that delays entry into metaphase."Nature 406:430-435(2000) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The finished DNA sequence of human chromosome 12." Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  6. "The checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transition."J. Cell Biol. 156:249-259(2002) [PubMed] [Europe PMC] [Abstract]
  7. "Chfr regulates a mitotic stress pathway through its RING-finger domain with ubiquitin ligase activity."Cancer Res. 62:1797-1801(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Aberrant hypermethylation of the CHFR prophase checkpoint gene in human lung cancers."Oncogene 21:2328-2333(2002) [PubMed] [Europe PMC] [Abstract]
  9. "Frequent hypermethylation of the 5' CpG island of the mitotic stress checkpoint gene Chfr in colorectal and non-small cell lung cancer."Carcinogenesis 24:47-51(2003) [PubMed] [Europe PMC] [Abstract]
  10. "Epigenetic inactivation of CHFR in human tumors."Proc. Natl. Acad. Sci. U.S.A. 100:7818-7823(2003) [PubMed] [Europe PMC] [Abstract]
  11. "Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer."Cancer Res. 63:8606-8613(2003) [PubMed] [Europe PMC] [Abstract]
  12. "Promotion of mitosis by activated protein kinase B after DNA damage involves polo-like kinase 1 and checkpoint protein CHFR."Mol. Cancer Res. 1:959-969(2003) [PubMed] [Europe PMC] [Abstract]
  13. "The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains."Oncogene 22:7101-7107(2003) [PubMed] [Europe PMC] [Abstract]
  14. "CHFR-associated early G2/M checkpoint defects in breast cancer cells."Mol. Carcinog. 39:26-33(2004) [PubMed] [Europe PMC] [Abstract]
  15. "PML bodies control the nuclear dynamics and function of the CHFR mitotic checkpoint protein."Nat. Struct. Mol. Biol. 11:1114-1121(2004) [PubMed] [Europe PMC] [Abstract]
  16. "Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins."Nature 451:81-85(2008) [PubMed] [Europe PMC] [Abstract]
  17. "The anti-proliferative effects of the CHFR depend on the forkhead associated domain, but not E3 ligase activity mediated by ring finger domain."PLoS ONE 3:E1776-E1776(2008) [PubMed] [Europe PMC] [Abstract]
  18. "Chfr is linked to tumour metastasis through the downregulation of HDAC1."Nat. Cell Biol. 11:295-302(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Crystal structure of the FHA domain of the Chfr mitotic checkpoint protein and its complex with tungstate."Structure 10:891-899(2002) [PubMed] [Europe PMC] [Abstract]
  20. "Inactivating mutations targeting the chfr mitotic checkpoint gene in human lung cancer."Cancer Res. 63:7185-7189(2003) [PubMed] [Europe PMC] [Abstract]