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Cleavage And Polyadenylation Specific Factor 3 (CPSF3)

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CPSF; YSH1; CPSF73; Cleavage and polyadenylation specificity factor 73 kDa subunit; mRNA 3'-end-processing endonuclease CPSF-73

Cleavage And Polyadenylation Specific Factor 3 (CPSF3)
CCC J350
HGNC 2326
MGI 1859328
PubMed 8929409
RGD 1305767
UniProt Q9UKF6
Wiki CPSF3
A poly(A) tail is added to eukaryotic mRNA precursors after tightly coupled cleavage and polyadenylation reactions at their 3-prime ends. The factor responsible for this activity, CPSF, contains multiple subunits (e.g., CPSF1).the crystal structures of human CPSF73 at 2.1-angstrom resolution, complexed with zinc ions and a sulfate that might mimic the phosphate group of substrate, and the related yeast protein CPSF100 (CPSF2) at 2.5-angstrom resolution. Both CPSF73 and CPSF100 contain 2 domains, a metallo-beta-lactamase domain and a novel beta-CASP domain. The active site of CPSF73, with 2 zinc ions, is located at the interface of the 2 domains. Purified recombinant CPSF73 possesses RNA endonuclease activity, and mutations that disrupt zinc binding in the active site abolish this activity.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cleavage And Polyadenylation Specific Factor 3 (CPSF3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 3 (CPSF3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 3 (CPSF3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cleavage And Polyadenylation Specific Factor 3 (CPSF3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cleavage And Polyadenylation Specific Factor 3 (CPSF3) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cleavage And Polyadenylation Specific Factor 3 (CPSF3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 3 (CPSF3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 3 (CPSF3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cleavage And Polyadenylation Specific Factor 3 (CPSF3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cleavage And Polyadenylation Specific Factor 3 (CPSF3) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cleavage And Polyadenylation Specific Factor 3 (CPSF3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 3 (CPSF3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 3 (CPSF3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cleavage And Polyadenylation Specific Factor 3 (CPSF3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cleavage And Polyadenylation Specific Factor 3 (CPSF3) ELISA Kit Customized Service Offer
  1. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase."EMBO J. 23:616-626(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease."RNA 10:565-573(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Sumoylation modulates the assembly and activity of the pre-mRNA 3' processing complex."Mol. Cell. Biol. 27:8848-8858(2007) [PubMed] [Europe PMC] [Abstract]
  6. "Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3'-end maturation."EMBO Rep. 9:1013-1018(2008) [PubMed] [Europe PMC] [Abstract]
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  8. "Molecular architecture of the human pre-mRNA 3' processing complex."Mol. Cell 33:365-376(2009) [PubMed] [Europe PMC] [Abstract]
  9. "The poly A polymerase Star-PAP controls 3'-end cleavage by promoting CPSF interaction and specificity toward the pre-mRNA."EMBO J. 29:4132-4145(2010) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  11. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  12. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  13. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  14. "Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease."Nature 444:953-956(2006) [PubMed] [Europe PMC] [Abstract]
  15. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]