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Cleavage And Polyadenylation Specific Factor 2 (CPSF2)

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CPSF100; Cleavage and polyadenylation specificity factor 100 kDa subunit

Cleavage And Polyadenylation Specific Factor 2 (CPSF2)
CCC J349
HGNC 2325
MGI 1861601
PubMed 22838834
RGD 1309687
UniProt Q9P2I0
Wiki CPSF2
A poly(A) tail is added to eukaryotic mRNA precursors after tightly coupled cleavage and polyadenylation reactions at their 3-prime ends. The factor responsible for this activity, CPSF, contains multiple subunits.
Northern blot analysis using bovine Cpsf2 coding sequences revealed expression of 5.6-, 4.6-, 3.8-, and 3.0-kb transcripts in HeLa cells. Immunoblot analysis showed expression of a 100-kD nuclear protein in bovine and human cells. Immunofluorescence microscopy demonstrated expression of both the 100-kD and the 160-kD (CPSF1) CPSF subunits in nucleoplasm but not in nucleoli.The partial human protein is 99% identical to the 782-amino acid bovine protein. RT-PCR analysis revealed ubiquitous but variable expression of CPSF2, with highest levels in liver and testis.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cleavage And Polyadenylation Specific Factor 2 (CPSF2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 2 (CPSF2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 2 (CPSF2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cleavage And Polyadenylation Specific Factor 2 (CPSF2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cleavage And Polyadenylation Specific Factor 2 (CPSF2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cleavage And Polyadenylation Specific Factor 2 (CPSF2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 2 (CPSF2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 2 (CPSF2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cleavage And Polyadenylation Specific Factor 2 (CPSF2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cleavage And Polyadenylation Specific Factor 2 (CPSF2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cleavage And Polyadenylation Specific Factor 2 (CPSF2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 2 (CPSF2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 2 (CPSF2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cleavage And Polyadenylation Specific Factor 2 (CPSF2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cleavage And Polyadenylation Specific Factor 2 (CPSF2) ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
  4. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  5. "Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase."EMBO J. 23:616-626(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  7. "Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3'-end maturation."EMBO Rep. 9:1013-1018(2008) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  11. "The poly A polymerase Star-PAP controls 3'-end cleavage by promoting CPSF interaction and specificity toward the pre-mRNA."EMBO J. 29:4132-4145(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  13. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]