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Epsin 1 (EPN1)

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EPS-15-Interacting Protein 1; EH domain-binding mitotic phosphoprotein

Epsin 1 (EPN1)
CCC J141
HGNC 21604
MGI 1333763
PubMed 9723620
RGD 619772
UniProt Q9Y6I3
Wiki EPN1
EPN1 is an endocytic accessory protein that interacts with EPS15, the alpha subunit of the clathrin adaptor AP2 (AP2A1), and clathrin, as well as with other accessory proteins for the endocytosis of clathrin-coated vesicles
The deduced 576-amino acid protein contains several conserved asp-pro-trp (DPW) repeats in its central region, a Cdc2 phosphorylation site, and 3 arg-pro-phe (NPF) repeats in its C-terminal region. Northern blot analysis detected a 2.6-kb transcript in all rat tissues tested, and Western blot analysis confirmed ubiquitous expression. Immunofluorescence localization using rat brain frozen sections revealed colocalization of Epn1 with Eps15, synaptophysin, and clathrin in nerve terminals.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ141Hu01 Recombinant Epsin 1 (EPN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAJ141Hu01 Polyclonal Antibody to Epsin 1 (EPN1) WB,IHC
Assay Kits n/a CLIA Kit for Epsin 1 (EPN1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Epsin 1 (EPN1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Epsin 1 (EPN1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Epsin 1 (EPN1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Epsin 1 (EPN1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Epsin 1 (EPN1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Epsin 1 (EPN1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Epsin 1 (EPN1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Epsin 1 (EPN1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Epsin 1 (EPN1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Epsin 1 (EPN1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Epsin 1 (EPN1) ELISA Kit Customized Service Offer
  1. "Epsin binds to the EH domain of POB1 and regulates receptor-mediated endocytosis."Oncogene 18:5915-5922(1999) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Regulation of complex formation of POB1/epsin/adaptor protein complex 2 by mitotic phosphorylation."J. Biol. Chem. 275:18399-18406(2000) [PubMed] [Europe PMC] [Abstract]
  6. "RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis."J. Biol. Chem. 278:30597-30604(2003) [PubMed] [Europe PMC] [Abstract]
  7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Molecular switches involving the AP-2 beta2 appendage regulate endocytic cargo selection and clathrin coat assembly."Dev. Cell 10:329-342(2006) [PubMed] [Europe PMC] [Abstract]
  9. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
  11. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  14. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  15. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  16. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  17. "Solution structure of the epsin N-terminal homology (ENTH) domain of human epsin."J. Struct. Funct. Genomics 2:1-8(2002) [PubMed] [Europe PMC] [Abstract]