Primary culture of rat aortic vascular smooth muscle cells (VSMCs) is extensively used in vitro to study the physiology and pathophysiology of cardiovascular diseases, including atherosclerosis (AS), hypertension, and aneurysm. The 2 methods mainly used to obtain primary culture of VSMCs are tissue explants method and enzyme digestion method.
Cells began to grow out at about 8 days and became relatively confluent within 16 days. Compared with VSMCs from the traditional tissue explants method and enzyme digestion method or A7r5 cell line, VSMCs obtained by our method showed higher purity and manifested a more “contractile” phenotype characteristic.
Organism species: Rattus norvegicus (Rat)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Cells | CSI380Ra11 | Rat A7r5 Aortic Vascular Smooth Muscle Cells (A7r5) | For research use only. It is not approved for human or animal use, or for applications in in vitro diganostic procedures. |
Extracts | n/a | Extract of A7r5 Aortic Vascular Smooth Muscle Cells (A7r5) | Total Protein/DNA/RNA Extract Customized Service Offer |
Mediums | MSI380Ra11 | Medium for Rat A7r5 Aortic Vascular Smooth Muscle Cells (A7r5) | Complete Medium for the culture of A7r5 cell line |