Editing A7r5 Aortic Vascular Smooth Muscle Cells(A7r5)

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Summary	<p><span >Primary culture of rat aortic vascular smooth muscle cells (VSMCs) is extensively used </span><em >in vitro</em><span > to study the physiology and pathophysiology of cardiovascular diseases, including atherosclerosis (AS), hypertension, and aneurysm</span><span >. The 2 methods mainly used to obtain primary culture of VSMCs are tissue explants method and enzyme digestion method</span><span >.</span></p><p><span ></span><span >Cells began to grow out at about 8 days and became relatively confluent within 16 days. Compared with VSMCs from the traditional tissue explants method and enzyme digestion method or A7r5 cell line, VSMCs obtained by our method showed higher purity and manifested a more “contractile” phenotype characteristic.</span><span ></span></p>
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