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Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1)

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GCF2; FLAP-1; FLIIAP1; GCF-2; HUFI-1; TRIP; GC-Binding Factor 2; TAR RNA-interacting protein

Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1)
CCC H718
HGNC 6702
MGI 1342770
PubMed 16199883
UniProt Q32MZ4
Wiki LRRFIP1
The GCF2 cDNA contains an internal region with 98% nucleotide identity with the first 309 bp of the GCF cDNA, but the remainder of the 2 sequences share no significant homology.
The predicted 752-amino acid GCF2 protein has a pI of 4.4. The authors confirmed the calculated molecular mass of 83 kD by mass spectroscopy. However, GCF2 migrates as a 160-kD protein by SDS-PAGE. the discrepancy between the calculated and observed masses is due either to the acidic nature of the protein or to an unusual ability of GCF2 to form very stable dimers. GCF2 binds to EGFR promoter fragments in vitro. Cotransfection assays showed that GCF2 represses transcription from 3 different promoters, including that of EGFR.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH718Hu01 Recombinant Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) Positive Control; Immunogen; SDS-PAGE; WB.
RPH718Hu02 Recombinant Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH718Hu01 Polyclonal Antibody to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) WB; IHC; ICC; IP.
PAH718Hu02 Polyclonal Antibody to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) WB; IHC; ICC; IP.
LAH718Hu71 Biotin-Linked Polyclonal Antibody to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) WB; IHC; ICC.
Assay Kits SEH718Hu ELISA Kit for Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) ELISA Kit Customized Service Offer
  1. "Molecular cloning and characterization of a transcription regulator with homology to GC-binding factor."J. Biol. Chem. 273:21594-21602(1998) [PubMed] [Europe PMC] [Abstract]
  2. "TRIP: a novel double stranded RNA binding protein which interacts with the leucine rich repeat of flightless I."Nucleic Acids Res. 26:3460-3467(1998) [PubMed] [Europe PMC] [Abstract]
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Novel proteins interacting with the leucine-rich repeat domain of human flightless-I identified by the yeast two-hybrid system."Genomics 58:146-157(1999) [PubMed] [Europe PMC] [Abstract]
  6. "GC factor 2 represses platelet-derived growth factor A-chain gene transcription and is itself induced by arterial injury."Circ. Res. 84:1258-1267(1999) [PubMed] [Europe PMC] [Abstract]
  7. "GCF2: expression and molecular analysis of repression."Biochim. Biophys. Acta 1629:15-25(2003) [PubMed] [Europe PMC] [Abstract]
  8. "GCF2/LRRFIP1 represses tumor necrosis factor alpha expression."Mol. Cell. Biol. 25:9073-9081(2005) [PubMed] [Europe PMC] [Abstract]
  9. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  10. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  11. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Modulation of TLR signaling by multiple MyD88-interacting partners including leucine-rich repeat Fli-I-interacting proteins."J. Immunol. 182:3450-3460(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  16. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  18. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  19. "Crystal structure of the dimeric coiled-coil domain of the cytosolic nucleic acid sensor LRRFIP1."J. Struct. Biol. 181:82-88(2013) [PubMed] [Europe PMC] [Abstract]
  20. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]