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Myosin Phosphatase Rho Interacting Protein (MPRIP)

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M-RIP; MRIP; RHOIP3; p116Rip; Rho Interacting Protein 3

Myosin Phosphatase Rho Interacting Protein (MPRIP)
CCC H704
HGNC 30321
MGI 1349438
PubMed 24162815
RGD 619947
UniProt Q6WCQ1
Wiki M-RIP
The deduced 1,024-amino acid protein has a calculated molecular mass of 116.4 kD. It has 2 proline-rich sequences near the N-terminus that are predicted to bind SH3 domains, a central pleckstrin homology (PH) domain, and a C-terminal coiled-coil region, which overlaps with the RhoA-binding domain.
It also has sites for myristoylation and phosphorylation, and it has a putative nuclear localization signal. Immunohistochemical analysis of coronary artery smooth muscle cells showed that MRIP colocalized with MBS and RhoA at actin stress fibers. MRIP did not show nuclear localization. Western blot analysis detected endogenous MRIP at an apparent molecular mass of 125 kD in human smooth muscle cell lines.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Myosin Phosphatase Rho Interacting Protein (MPRIP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Myosin Phosphatase Rho Interacting Protein (MPRIP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Myosin Phosphatase Rho Interacting Protein (MPRIP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Myosin Phosphatase Rho Interacting Protein (MPRIP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Myosin Phosphatase Rho Interacting Protein (MPRIP) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Myosin Phosphatase Rho Interacting Protein (MPRIP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Myosin Phosphatase Rho Interacting Protein (MPRIP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Myosin Phosphatase Rho Interacting Protein (MPRIP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Myosin Phosphatase Rho Interacting Protein (MPRIP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Myosin Phosphatase Rho Interacting Protein (MPRIP) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Myosin Phosphatase Rho Interacting Protein (MPRIP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Myosin Phosphatase Rho Interacting Protein (MPRIP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Myosin Phosphatase Rho Interacting Protein (MPRIP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Myosin Phosphatase Rho Interacting Protein (MPRIP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Myosin Phosphatase Rho Interacting Protein (MPRIP) ELISA Kit Customized Service Offer
  1. "Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA."J. Biol. Chem. 278:51484-51493(2003) [PubMed] [Europe PMC] [Abstract].
  2. "p116Rip decreases myosin II phosphorylation by activating myosin light chain phosphatase and by inactivating RhoA."J. Biol. Chem. 280:4983-4991(2005) [PubMed] [Europe PMC] [Abstract]
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract], Lymph and Muscle.
  6. "M-RIP targets myosin phosphatase to stress fibers to regulate myosin light chain phosphorylation in vascular smooth muscle cells."J. Biol. Chem. 280:42543-42551(2005) [PubMed] [Europe PMC] [Abstract]
  7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  9. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]