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Mannose Phosphate Isomerase (MPI)

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PMI1; Mannose-6-Phosphate Isomerase; Phosphohexomutase; Phosphomannose isomerase

Mannose Phosphate Isomerase (MPI)
CCC H620
HGNC 7216
MGI 97075
PubMed 16488169
RGD 3107
UniProt P34949
Wiki MPI
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. The authors used sequence information obtained from internal fragments of the protein to design degenerate oligonucleotides which were used to amplify a fragment of the PMI cDNA.The PMI gene encodes a predicted 423-amino acid polypeptide. Northern blot analysis detected a single 1.8-kb PMI mRNA in all tissues tested, with the highest levels in heart, brain, and skeletal muscle. Recombinant PMI expressed in E. coli had very similar activity to the native enzyme.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH620Hu01 Recombinant Mannose Phosphate Isomerase (MPI) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAH620Hu21 Monoclonal Antibody to Mannose Phosphate Isomerase (MPI) WB; IHC; ICC; IP.
PAH620Hu01 Polyclonal Antibody to Mannose Phosphate Isomerase (MPI) WB,IHC
Assay Kits SEH620Hu ELISA Kit for Mannose Phosphate Isomerase (MPI) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mannose Phosphate Isomerase (MPI) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mannose Phosphate Isomerase (MPI) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mannose Phosphate Isomerase (MPI) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mannose Phosphate Isomerase (MPI) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mannose Phosphate Isomerase (MPI) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mannose Phosphate Isomerase (MPI) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mannose Phosphate Isomerase (MPI) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mannose Phosphate Isomerase (MPI) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mannose Phosphate Isomerase (MPI) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mannose Phosphate Isomerase (MPI) ELISA Kit Customized Service Offer
  1. "Purification, cDNA cloning and heterologous expression of human phosphomannose isomerase."Eur. J. Biochem. 219:415-423(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Genomic organization of the human phosphomannose isomerase (MPI) gene and mutation analysis in patients with congenital disorders of glycosylation type Ib (CDG-Ib)."Hum. Mutat. 16:247-252(2000) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  6. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  8. "Phosphomannose isomerase deficiency: a carbohydrate-deficient glycoprotein syndrome with hepatic-intestinal presentation."Am. J. Hum. Genet. 62:1535-1539(1998) [PubMed] [Europe PMC] [Abstract]
  9. "Carbohydrate-deficient glycoprotein syndrome type Ib: phosphomannose isomerase deficiency and mannose therapy."J. Clin. Invest. 101:1414-1420(1998) [PubMed] [Europe PMC] [Abstract]
  10. "A broad spectrum of clinical presentations in congenital disorders of glycosylation I: a series of 26 cases." J. Med. Genet. 38:14-19(2001) [PubMed] [Europe PMC] [Abstract]
  11. "Genetic and metabolic analysis of the first adult with congenital disorder of glycosylation type Ib: long-term outcome and effects of mannose supplementation."Mol. Genet. Metab. 73:77-85(2001) [PubMed] [Europe PMC] [Abstract]
  12. "DHPLC analysis as a platform for molecular diagnosis of congenital disorders of glycosylation (CDG)."Eur. J. Hum. Genet. 10:643-648(2002) [PubMed] [Europe PMC] [Abstract]
  13. "Protein losing enteropathy-hepatic fibrosis syndrome in Saguenay-Lac St-Jean, Quebec is a congenital disorder of glycosylation type Ib."J. Med. Genet. 39:849-851(2002) [PubMed] [Europe PMC] [Abstract]