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Pinin (PNN)

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DRS; SDK3; MEMA; Desmosome Associated Protein; Domain-rich serine protein; Melanoma metastasis clone A protein; 140 kDa nuclear and cell adhesion-related phosphoprotein

Pinin (PNN)
CCC H341
HGNC 9162
MGI 1100514
PubMed 10645008
RGD 1597086
UniProt Q9H307
Wiki Pinin
PNN protein contains a serine-rich domain; a glutamine-proline, glutamine-leucine repeat domain; an acidic domain rich in glutamic acid; and numerous potential kinase recognition motifs. Northern blot analysis detected ubiquitous expression of PNN in human tissues, including those lacking desmosomes. PNN is expressed as 4.1-, 3.7-, and 3.2-kb transcripts that show tissue-specific expression patterns. Southern blot analysis demonstrated the existence of a single pinin gene in the human genome. Since the 3-prime end of the PNN coding sequence is nearly identical to a partial cDNA identified in a pig neutrophil cDNA library, and since leukocytes, which do not react with antibodies against pinin, express an mRNA that hybridizes with a pinin cDNA, the authors speculated that there may be a pinin-related gene.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Pinin (PNN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Pinin (PNN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Pinin (PNN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Pinin (PNN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Pinin (PNN) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Pinin (PNN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Pinin (PNN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Pinin (PNN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Pinin (PNN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Pinin (PNN) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Pinin (PNN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Pinin (PNN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Pinin (PNN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Pinin (PNN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Pinin (PNN) ELISA Kit Customized Service Offer
  1. "Characterization of pinin, a novel protein associated with the desmosome-intermediate filament complex."J. Cell Biol. 135:1027-1042(1996) [PubMed] [Europe PMC] [Abstract]
  2. "memA/DRS, a putative mediator of multiprotein complexes, is overexpressed in the metastasizing human melanoma cell lines BLM and MV3."Biochim. Biophys. Acta 1444:384-394(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Characterization of the gene encoding pinin/DRS/memA and evidence for its potential tumor suppressor function."Oncogene 19:289-297(2000) [PubMed] [Europe PMC] [Abstract]
  4. "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Evidence that 'pinin', reportedly a differentiation-specific desmosomal protein, is actually a widespread nuclear protein."Differentiation 62:119-127(1997) [PubMed] [Europe PMC] [Abstract]
  8. , Submitted (MAY-2006) to UniProtKB
  9. "Antibodies differentiate desmosome-form and nucleus-form pinin: evidence that pinin is a moonlighting protein with dual location at the desmosome and within the nucleus."Biochem. Biophys. Res. Commun. 263:192-200(1999) [PubMed] [Europe PMC] [Abstract]
  10. "Dissection of protein linkage between keratins and pinin, a protein with dual location at desmosome-intermediate filament complex and in the nucleus."J. Biol. Chem. 275:14910-14915(2000) [PubMed] [Europe PMC] [Abstract]
  11. "Modulation of alternative pre-mRNA splicing in vivo by pinin."Biochem. Biophys. Res. Commun. 294:448-455(2002) [PubMed] [Europe PMC] [Abstract]
  12. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
  13. "Molecular characterization of a novel nucleolar protein, pNO40."Biochem. Biophys. Res. Commun. 307:569-577(2003) [PubMed] [Europe PMC] [Abstract]
  14. "Pinin/DRS/memA interacts with SRp75, SRm300 and SRrp130 in corneal epithelial cells."Invest. Ophthalmol. Vis. Sci. 44:4715-4723(2003) [PubMed] [Europe PMC] [Abstract]
  15. "Nuclear Pnn/DRS protein binds to spliced mRNPs and participates in mRNA processing and export via interaction with RNPS1."Mol. Cell. Biol. 23:7363-7376(2003) [PubMed] [Europe PMC] [Abstract]
  16. "Over-expression of SR-cyclophilin, an interaction partner of nuclear pinin, releases SR family splicing factors from nuclear speckles."Biochem. Biophys. Res. Commun. 321:638-647(2004) [PubMed] [Europe PMC] [Abstract]
  17. "Human RNPS1 and its associated factors: a versatile alternative pre-mRNA splicing regulator in vivo."Mol. Cell. Biol. 24:1174-1187(2004) [PubMed] [Europe PMC] [Abstract]
  18. "Nuclear speckle-associated protein Pnn/DRS binds to the transcriptional corepressor CtBP and relieves CtBP-mediated repression of the E-cadherin gene."Mol. Cell. Biol. 24:10223-10235(2004) [PubMed] [Europe PMC] [Abstract]
  19. "Reduction of Pnn by RNAi induces loss of cell-cell adhesion between human corneal epithelial cells."Mol. Vis. 11:133-142(2005) [PubMed] [Europe PMC] [Abstract]
  20. "Biochemical analysis of the EJC reveals two new factors and a stable tetrameric protein core."RNA 11:1869-1883(2005) [PubMed] [Europe PMC] [Abstract]
  21. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  22. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  23. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  24. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  25. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  27. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  28. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  29. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  30. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  31. "The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex."Nat. Struct. Mol. Biol. 19:378-386(2012) [PubMed] [Europe PMC] [Abstract]
  32. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]