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Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1)

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FDG; HMUDG; UNG3

Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1)
CCC H164
HGNC 17148
MGI 1918976
PubMed 10074426
RGD 631403
UniProt Q53HV7
Wiki SMUG1
SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair. The deduced 270-amino acid protein has a calculated molecular mass of about 30 kD. Human SMUG1 shares 60% identity with the Xenopus protein. Fluorescence-tagged SMUG1 localized predominantly to the nucleus of transiently transfected HeLa cells.
SMUG1 showed broader substrate specificity than UNG2, and AP endonuclease had a strong stimulatory effect on SMUG1 against double-stranded uracil, apparently due to enhance dissociation of SMUG1 from AP sites in double-stranded DNA. SMUG1 accumulated within nucleoli in cultured epithelial cells, while UNG2 was excluded from nucleoli.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH164Hu01 Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH164Hu01 Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) WB; IHC; ICC; IP.
MAH164Hu21 Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) WB; IHC; ICC; IP.
Assay Kits SEH164Hu ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) ELISA Kit Customized Service Offer
  1. "Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors."Curr. Biol. 9:174-185(1999) [PubMed] [Europe PMC] [Abstract]
  2. "Mammalian 5-formyluracil-DNA glycosylase. 2. Role of SMUG1 uracil-DNA glycosylase in repair of 5-formyluracil and other oxidized and deaminated base lesions."Biochemistry 42:5003-5012(2003) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1."J. Biol. Chem. 276:41991-41997(2001) [PubMed] [Europe PMC] [Abstract]
  6. "hUNG2 is the major repair enzyme for removal of uracil from U:A matches, U:G mismatches, and U in single-stranded DNA, with hSMUG1 as a broad specificity backup."J. Biol. Chem. 277:39926-39936(2002) [PubMed] [Europe PMC] [Abstract]
  7. "Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase."Nucleic Acids Res. 32:5291-5302(2004) [PubMed] [Europe PMC] [Abstract]