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Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a)

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CT; CTPCT; PCYT1; CCT A; CCT-alpha; CTP:phosphocholine cytidylyltransferase A; Phosphorylcholine transferase A

Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a)
CCC G535
HGNC 8754
MGI 88557
PubMed 24387990
RGD 70515
UniProt P49585
Wiki PCYT1A
The rate-controlling enzyme in the phosphatidylcholine biosynthetic pathway is CTP-phosphocholine cytidylyltransferase (EC 2.7.7.15). In cultured eukaryotic cells, this enzyme is essential for survival. The alpha isoform is located in the nucleus and is regulated by reversible phosphorylation and membrane association.
The human nucleotide sequence, encoding a 367-amino acid polypeptide, is 89% identical to the rat nucleotide sequence. The catalytic domain of human CTPCT has homology to regions of Bacillus subtilis glycerol phosphate cytidylyltransferase and yeast MUQ1. Kalmar et al. (1994) observed significant identity between the alpha-helical membrane-binding domains of CTPCT and soybean oleosin. Expressed CTPCT had lipid-dependent cytidylyltransferase activity.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphate Cytidylyltransferase 1, Choline Alpha (PCYT1a) ELISA Kit Customized Service Offer
  1. "Primary structure and expression of a human CTP:phosphocholine cytidylyltransferase."Biochim. Biophys. Acta 1219:328-334(1994) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Membrane-binding amphipathic alpha-helical peptide derived from CTP:phosphocholine cytidylyltransferase."Biochemistry 33:4327-4335(1994) [PubMed] [Europe PMC] [Abstract]
  4. "Structure of the membrane binding domain of CTP:phosphocholine cytidylyltransferase."Biochemistry 35:11975-11984(1996) [PubMed] [Europe PMC] [Abstract]
  5. "Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase."J. Biol. Chem. 279:28817-28825(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  15. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  16. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  17. "Mutations in PCYT1A, encoding a key regulator of phosphatidylcholine metabolism, cause spondylometaphyseal dysplasia with cone-rod dystrophy."Am. J. Hum. Genet. 94:105-112(2014) [PubMed] [Europe PMC] [Abstract]
  18. "Mutations in PCYT1A cause spondylometaphyseal dysplasia with cone-rod dystrophy."Am. J. Hum. Genet. 94:113-119(2014) [PubMed] [Europe PMC] [Abstract]