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Replication Initiator 1 (REPIN1)

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AP4; RIP60; ZNF464; Zfp464; Replication Initiation Region Protein(60kD); Zinc Finger Protein 464; 60 kDa origin-specific DNA-binding protein; ATT-binding protein

Replication Initiator 1 (REPIN1)
CCC G516
HGNC 17922
MGI 1889817
PubMed 21701954
RGD 1549707
UniProt Q9BWE0
Wiki REPIN1
Replication initiator 1 is a protein encoded by the REPIN1 gene. Homodimers of RIP60 (replication initiation-region protein 60 kDA) purified from nuclear extract bind two ATT-rich sites in oribeta and foster the formation of a twisted 720 bp DNA loop in vitro.
RIP60 contains 15 C(2)H(2)zinc finger (ZF) DNA binding motifs organized in three clusters, termed hand Z1 (ZFs 1-5), hand Z2 (ZFs 6-8) and hand Z3 (ZFs 9-15). A proline-rich region is located between hands Z2 and Z3. Gel mobility shift and DNase I footprinting experiments show hands Z1 and Z2 independently bind the oribeta RIP60 sites specifically, but with different affinities. Hand Z3 binds DNA, but displays no specificity for RIP60 sites.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPG516Hu01 Recombinant Replication Initiator 1 (REPIN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAG516Hu01 Polyclonal Antibody to Replication Initiator 1 (REPIN1) WB; IHC; ICC; IP.
MAG516Hu21 Monoclonal Antibody to Replication Initiator 1 (REPIN1) WB; IHC; ICC; IP.
Assay Kits SEG516Hu ELISA Kit for Replication Initiator 1 (REPIN1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPG516Mu01 Recombinant Replication Initiator 1 (REPIN1) Positive Control; Immunogen; SDS-PAGE; WB.
RPG516Mu02 Recombinant Replication Initiator 1 (REPIN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAG516Mu01 Polyclonal Antibody to Replication Initiator 1 (REPIN1) WB; IHC; ICC; IP.
PAG516Mu02 Polyclonal Antibody to Replication Initiator 1 (REPIN1) WB; IHC; ICC; IP.
Assay Kits SEG516Mu ELISA Kit for Replication Initiator 1 (REPIN1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Replication Initiator 1 (REPIN1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Replication Initiator 1 (REPIN1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Replication Initiator 1 (REPIN1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Replication Initiator 1 (REPIN1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Replication Initiator 1 (REPIN1) ELISA Kit Customized Service Offer
  1. "The dhfr oribeta-binding protein RIP60 contains 15 zinc fingers: DNA binding and looping by the central three fingers and an associated proline-rich region."Nucleic Acids Res. 28:570-581(2000) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence of human chromosome 7." Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Purification of RIP60 and RIP100, mammalian proteins with origin-specific DNA-binding and ATP-dependent DNA helicase activities."Mol. Cell. Biol. 10:6225-6235(1990) [PubMed] [Europe PMC] [Abstract]
  5. "RIP60, a mammalian origin-binding protein, enhances DNA bending near the dihydrofolate reductase origin of replication."Mol. Cell. Biol. 10:6236-6243(1990) [PubMed] [Europe PMC] [Abstract]
  6. "RIP60 dimers and multiples of dimers assemble link structures at an origin of bidirectional replication in the dihydrofolate reductase amplicon of Chinese hamster ovary cells."J. Mol. Biol. 232:766-778(1993) [PubMed] [Europe PMC] [Abstract]
  7. "Condensation by DNA looping facilitates transfer of large DNA molecules into mammalian cells."Nucleic Acids Res. 29:1982-1988(2001) [PubMed] [Europe PMC] [Abstract]
  8. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  9. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]