Double C2 Like Domains Alpha (DOC2a)

C2-like domains that interact with Ca(2+) and phospholipid are found in synaptotagmin and rabphilin-3A (RPH3A), proteins implicated in neurotransmitter release. By screening a human brain cDNA library with a fragment showing homology to rabphilin-3A, Orita et al. (1995) isolated a novel cDNA which they named DOC2 for 'double C2.' The cDNA predicts a 400-amino acid protein with relative mass of 44,071 Da and 2 repeated C2-like domains in the C-terminal region. Northern blot analysis showed high levels of a 2.2-kb DOC2 transcript in brain. Expression of a recombinant DOC2 protein in E. coli showed that it interacts with Ca(2+) and phospholipid.The International Radiation Hybrid Mapping Consortium mapped the DOC2A gene to chromosome 16.