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Polymerase DNA Directed Kappa (POLk)

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DINB1; DINP

Polymerase DNA Directed Kappa (POLk)
CCC F726
HGNC 9183
MGI 1349767
PubMed 12760019
RGD 620058
UniProt Q9UBT6
Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass.
The deduced 870-amino acid POLK protein contains an N-terminal conserved nucleotidyl transferase domain, 2 tandem helix-hairpin-helix domains implicated in DNA binding, and a nonconserved C terminus containing 2 zinc clusters. Northern blot analysis revealed ubiquitous expression of a 5.0-kb transcript at low but varying levels in all tissues tested. Highest expression was found in testis, which also had abundant 3.2- and 4.4-kb transcripts.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polymerase DNA Directed Kappa (POLk) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polymerase DNA Directed Kappa (POLk) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polymerase DNA Directed Kappa (POLk) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polymerase DNA Directed Kappa (POLk) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polymerase DNA Directed Kappa (POLk) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polymerase DNA Directed Kappa (POLk) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polymerase DNA Directed Kappa (POLk) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polymerase DNA Directed Kappa (POLk) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polymerase DNA Directed Kappa (POLk) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polymerase DNA Directed Kappa (POLk) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polymerase DNA Directed Kappa (POLk) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polymerase DNA Directed Kappa (POLk) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polymerase DNA Directed Kappa (POLk) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polymerase DNA Directed Kappa (POLk) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polymerase DNA Directed Kappa (POLk) ELISA Kit Customized Service Offer
  1. "Mutation enhancement by DINB1, a mammalian homologue of the Escherichia coli mutagenesis protein dinB."Genes Cells 4:607-618(1999) [PubMed] [Europe PMC] [Abstract]
  2. "Human and mouse homologs of Escherichia coli DinB (DNA polymerase IV), members of the UmuC/DinB superfamily."Proc. Natl. Acad. Sci. U.S.A. 96:11922-11927(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Multiple PolK (POLK) transcripts in mammalian testis."DNA Repair 4:397-402(2005) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and comparative analysis of human chromosome 5." Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Purification and characterization of pol kappa, a DNA polymerase encoded by the human DINB1 gene."J. Biol. Chem. 276:92-98(2001) [PubMed] [Europe PMC] [Abstract]
  8. "Human DNA polymerase kappa bypasses and extends beyond thymine glycols during translesion synthesis in vitro, preferentially incorporating correct nucleotides."J. Biol. Chem. 277:37604-37611(2002) [PubMed] [Europe PMC] [Abstract]
  9. "Localisation of human DNA polymerase kappa to replication foci."J. Cell Sci. 115:4413-4418(2002) [PubMed] [Europe PMC] [Abstract]
  10. "Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA."Mol. Cell. Biol. 22:784-791(2002) [PubMed] [Europe PMC] [Abstract]
  11. "Role of human DNA polymerase kappa as an extender in translesion synthesis."Proc. Natl. Acad. Sci. U.S.A. 99:16000-16005(2002) [PubMed] [Europe PMC] [Abstract]
  12. "Human DNA polymerase kappa uses template-primer misalignment as a novel means for extending mispaired termini and for generating single-base deletions."Genes Dev. 17:2191-2199(2003) [PubMed] [Europe PMC] [Abstract]
  13. "A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair."Genes Dev. 17:2777-2785(2003) [PubMed] [Europe PMC] [Abstract]
  14. "Translesion synthesis past 2'-deoxyxanthosine, a nitric oxide-derived DNA adduct, by mammalian DNA polymerases."J. Mol. Biol. 344:665-674(2004) [PubMed] [Europe PMC] [Abstract]
  15. "Crystal structure of the catalytic core of human DNA polymerase kappa."Structure 12:1395-1404(2004) [PubMed] [Europe PMC] [Abstract]