Doublecortin Like Kinase 2 (DCLK2)

The deduced protein contains an N-terminal doublecortin (DCX) domain, followed by a serine-, threonine-, and proline-rich region and a C-terminal kinase domain. Northern blot analysis revealed that expression of Dck2 increased in mouse brain during the second half of embryonic development and remained high in adult brain.

Immunofluorescence confocal microscopy showed that Dck2 localized to both axons and dendrites of cultured rat sympathetic neurons. Downregulation of CRE-dependent gene activation was mediated by phosphorylated Torc2 (CRTC2) and retention of Torc2 in the cytoplasm. Overexpression of Cl2 also induced microtubule bundling in transfected HeLa cells and induced the development of aberrant multinucleated cells.