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Erythrocyte Membrane Protein Band 4.9 (EPB49)

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DMT; DMTN; Dematin; Dematin actin-binding protein

Erythrocyte Membrane Protein Band 4.9 (EPB49)
CCC F169
HGNC 3382
MGI 99670
PubMed 22927433
RGD 1311419
UniProt Q08495
Wiki EPB49
Dematin, or EPB49, is an actin-bundling protein originally identified in the erythroid membrane skeleton. Its actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase and is restored after dephosphorylation.
The deduced 383-amino acid protein has a calculated molecular mass of 43 kD. Dematin has a polyglutamine motif that may constitute a nuclear localization signal, a PEST sequence, a C-terminal domain highly similar to the C-terminal headpiece domain of villin (VIL), and several putative phosphorylation sites. Northern blot analysis detected variable dematin expression in all tissues examined. Immunofluorescence microscopy revealed punctate dematin staining around the cell nucleus in cultured human erythroblasts.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPF169Hu01 Recombinant Erythrocyte Membrane Protein Band 4.9 (EPB49) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Erythrocyte Membrane Protein Band 4.9 (EPB49) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Erythrocyte Membrane Protein Band 4.9 (EPB49) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Erythrocyte Membrane Protein Band 4.9 (EPB49) CLIA Kit Customized Service Offer
n/a ELISA Kit for Erythrocyte Membrane Protein Band 4.9 (EPB49) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Erythrocyte Membrane Protein Band 4.9 (EPB49) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Erythrocyte Membrane Protein Band 4.9 (EPB49) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Erythrocyte Membrane Protein Band 4.9 (EPB49) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Erythrocyte Membrane Protein Band 4.9 (EPB49) CLIA Kit Customized Service Offer
n/a ELISA Kit for Erythrocyte Membrane Protein Band 4.9 (EPB49) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Erythrocyte Membrane Protein Band 4.9 (EPB49) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Erythrocyte Membrane Protein Band 4.9 (EPB49) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Erythrocyte Membrane Protein Band 4.9 (EPB49) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Erythrocyte Membrane Protein Band 4.9 (EPB49) CLIA Kit Customized Service Offer
n/a ELISA Kit for Erythrocyte Membrane Protein Band 4.9 (EPB49) ELISA Kit Customized Service Offer
  1. "Cloning of human erythroid dematin reveals another member of the villin family."Proc. Natl. Acad. Sci. U.S.A. 90:6651-6655(1993) [PubMed] [Europe PMC] [Abstract]
  2. "Isoform cloning, actin binding, and chromosomal localization of human erythroid dematin, a member of the villin superfamily."J. Biol. Chem. 270:17407-17413(1995) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "DNA sequence and analysis of human chromosome 8." Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Loss of heterozygosity on 8p in prostate cancer implicates a role for dematin in tumor progression."Cancer Genet. Cytogenet. 115:65-69(1999) [PubMed] [Europe PMC] [Abstract]
  7. "Dematin interacts with the Ras-guanine nucleotide exchange factor Ras-GRF2 and modulates mitogen-activated protein kinase pathways."Eur. J. Biochem. 269:638-649(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Dematin and adducin provide a novel link between the spectrin cytoskeleton and human erythrocyte membrane by directly interacting with glucose transporter-1."J. Biol. Chem. 283:14600-14609(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Dematin exhibits a natively unfolded core domain and an independently folded headpiece domain."Protein Sci. 18:629-636(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  15. "Dematin, a component of the erythrocyte membrane skeleton, is internalized by the malaria parasite and associates with Plasmodium 14-3-3."J. Biol. Chem. 286:1227-1236(2011) [PubMed] [Europe PMC] [Abstract]
  16. "Identification of a novel role for dematin in regulating red cell membrane function by modulating spectrin-actin interaction."J. Biol. Chem. 287:35244-35250(2012) [PubMed] [Europe PMC] [Abstract]
  17. "Headpiece domain of dematin regulates calcium mobilization and signaling in platelets."J. Biol. Chem. 287:41218-41231(2012) [PubMed] [Europe PMC] [Abstract]
  18. "The allosteric mechanism induced by protein kinase A (PKA) phosphorylation of dematin (band 4.9)."J. Biol. Chem. 288:8313-8320(2013) [PubMed] [Europe PMC] [Abstract]
  19. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  20. "The NMR structure of dematin headpiece reveals a dynamic loop that is conformationally altered upon phosphorylation at a distal site."J. Biol. Chem. 279:7909-7916(2004) [PubMed] [Europe PMC] [Abstract]
  21. "A phosphorylation-induced conformation change in dematin headpiece."Structure 14:379-387(2006) [PubMed] [Europe PMC] [Abstract]