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Bromodomain Containing Protein 4 (BRD4)

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CAP; HUNKI; MCAP; HUNK1; Chromosome-Associated Protein

Bromodomain Containing Protein 4 (BRD4)
CCC F129
HGNC 13575
MGI 1888520
PubMed 23728299
RGD 1307282
UniProt O60885
Wiki BRD4
Bromodomain-containing protein 4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPF129Hu01 Recombinant Bromodomain Containing Protein 4 (BRD4) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Bromodomain Containing Protein 4 (BRD4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Bromodomain Containing Protein 4 (BRD4) Polyclonal Antibody Customized Service Offer
Assay Kits SEF129Hu ELISA Kit for Bromodomain Containing Protein 4 (BRD4) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Bromodomain Containing Protein 4 (BRD4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Bromodomain Containing Protein 4 (BRD4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Bromodomain Containing Protein 4 (BRD4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Bromodomain Containing Protein 4 (BRD4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Bromodomain Containing Protein 4 (BRD4) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Bromodomain Containing Protein 4 (BRD4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Bromodomain Containing Protein 4 (BRD4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Bromodomain Containing Protein 4 (BRD4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Bromodomain Containing Protein 4 (BRD4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Bromodomain Containing Protein 4 (BRD4) ELISA Kit Customized Service Offer
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  2. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "BRD4-NUT fusion oncogene: a novel mechanism in aggressive carcinoma."Cancer Res. 63:304-307(2003) [PubMed] [Europe PMC] [Abstract]
  5. "The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription."Mol. Cell 19:523-534(2005) [PubMed] [Europe PMC] [Abstract]
  6. "Recruitment of P-TEFb for stimulation of transcriptional elongation by the bromodomain protein Brd4."Mol. Cell 19:535-545(2005) [PubMed] [Europe PMC] [Abstract]
  7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen interacts with bromodomain protein Brd4 on host mitotic chromosomes."J. Virol. 80:8909-8919(2006) [PubMed] [Europe PMC] [Abstract]
  9. "ChlR1 is required for loading papillomavirus E2 onto mitotic chromosomes and viral genome maintenance."Mol. Cell 24:867-876(2006) [PubMed] [Europe PMC] [Abstract]
  10. "The EBNA1 protein of Epstein-Barr virus functionally interacts with Brd4."J. Virol. 82:12009-12019(2008) [PubMed] [Europe PMC] [Abstract]
  11. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Control of inducible gene expression by signal-dependent transcriptional elongation."Cell 138:129-145(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Brd4 coactivates transcriptional activation of NF-kappaB via specific binding to acetylated RelA."Mol. Cell. Biol. 29:1375-1387(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  17. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  18. "The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3."Mol. Cell. Biol. 31:2641-2652(2011) [PubMed] [Europe PMC] [Abstract]
  19. "Signal-induced Brd4 release from chromatin is essential for its role transition from chromatin targeting to transcriptional regulation."Nucleic Acids Res. 39:9592-9604(2011) [PubMed] [Europe PMC] [Abstract]
  20. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  21. "Bromodomain protein Brd4 associated with acetylated chromatin is important for maintenance of higher-order chromatin structure."J. Biol. Chem. 287:10738-10752(2012) [PubMed] [Europe PMC] [Abstract]
  22. "Bromodomain-containing protein 4 (BRD4) regulates RNA polymerase II serine 2 phosphorylation in human CD4+ T cells."J. Biol. Chem. 287:43137-43155(2012) [PubMed] [Europe PMC] [Abstract]
  23. "BRD4 is an atypical kinase that phosphorylates serine2 of the RNA polymerase II carboxy-terminal domain."Proc. Natl. Acad. Sci. U.S.A. 109:6927-6932(2012) [PubMed] [Europe PMC] [Abstract]
  24. "Phospho switch triggers Brd4 chromatin binding and activator recruitment for gene-specific targeting."Mol. Cell 49:843-857(2013) [PubMed] [Europe PMC] [Abstract]
  25. "BRD4 coordinates recruitment of pause release factor P-TEFb and the pausing complex NELF/DSIF to regulate transcription elongation of interferon-stimulated genes."Mol. Cell. Biol. 33:2497-2507(2013) [PubMed] [Europe PMC] [Abstract]
  26. "The bromodomain protein Brd4 insulates chromatin from DNA damage signalling." Nature 498:246-250(2013) [PubMed] [Europe PMC] [Abstract]
  27. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  28. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
  29. "Structural basis and binding properties of the second bromodomain of Brd4 with acetylated histone tails."Biochemistry 47:6403-6417(2008) [PubMed] [Europe PMC] [Abstract]
  30. "Selective inhibition of BET bromodomains." Nature 468:1067-1073(2010) [PubMed] [Europe PMC] [Abstract]
  31. "Suppression of inflammation by a synthetic histone mimic."Nature 468:1119-1123(2010) [PubMed] [Europe PMC] [Abstract]
  32. "Discovery and characterization of small molecule inhibitors of the BET family bromodomains." J. Med. Chem. 54:3827-3838(2011) [PubMed] [Europe PMC] [Abstract]
  33. "Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia." Nature 478:529-533(2011) [PubMed] [Europe PMC] [Abstract]
  34. "Benzodiazepines and benzotriazepines as protein interaction inhibitors targeting bromodomains of the BET family."Bioorg. Med. Chem. 20:1878-1886(2012) [PubMed] [Europe PMC] [Abstract]
  35. "Down-regulation of NF-kappaB transcriptional activity in HIV-associated kidney disease by BRD4 inhibition."J. Biol. Chem. 287:28840-28851(2012) [PubMed] [Europe PMC] [Abstract]
  36. "Fragment-based discovery of bromodomain inhibitors part 1: inhibitor binding modes and implications for lead discovery."J. Med. Chem. 55:576-586(2012) [PubMed] [Europe PMC] [Abstract]
  37. "Identification of a chemical probe for bromo and extra C-terminal bromodomain inhibition through optimization of a fragment-derived hit."J. Med. Chem. 55:9831-9837(2012) [PubMed] [Europe PMC] [Abstract]
  38. "Histone recognition and large-scale structural analysis of the human bromodomain family."Cell 149:214-231(2012) [PubMed] [Europe PMC] [Abstract]
  39. "Optimization of 3,5-dimethylisoxazole derivatives as potent bromodomain ligands."J. Med. Chem. 56:3217-3227(2013) [PubMed] [Europe PMC] [Abstract]
  40. "Fragment-based drug discovery of 2-thiazolidinones as inhibitors of the histone reader BRD4 bromodomain."J. Med. Chem. 56:3833-3851(2013) [PubMed] [Europe PMC] [Abstract]