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G Protein Alpha 12 (GNa12)

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RMP; NNX3; Gep; Guanine Nucleotide-Binding Protein Subunit Alpha-12

G Protein Alpha 12 (GNa12)
CCC F062
HGNC 4380
MGI 95767
PubMed 18703424
RGD 71018
UniProt Q03113
Wiki GNA12
The predicted GNA12 protein contains 381 amino acids.Protein pull-down assays confirmed that G-alpha-12 interacted directly with alpha-SNAP in COS-7 and human umbilical vein endothelial cells (HUVECs). Domain-swapping experiments and mutation analysis showed that the first 37 N-terminal amino acids of G-alpha-12 and the convex surface of alpha-SNAP were necessary for the interaction. Cotransfection of HUVECs with G-alpha-12 and alpha-SNAP stabilized VE-cadherin (CDH5) at the plasma membrane, whereas downregulation of alpha-SNAP with small interfering RNA resulted in loss of VE-cadherin from the cell surface. Downregulation of alpha-SNAP in conjunction with G-alpha-12 overexpression decreased HUVEC endothelial barrier function.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant G Protein Alpha 12 (GNa12) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to G Protein Alpha 12 (GNa12) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to G Protein Alpha 12 (GNa12) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for G Protein Alpha 12 (GNa12) CLIA Kit Customized Service Offer
n/a ELISA Kit for G Protein Alpha 12 (GNa12) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant G Protein Alpha 12 (GNa12) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to G Protein Alpha 12 (GNa12) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to G Protein Alpha 12 (GNa12) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for G Protein Alpha 12 (GNa12) CLIA Kit Customized Service Offer
n/a ELISA Kit for G Protein Alpha 12 (GNa12) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant G Protein Alpha 12 (GNa12) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to G Protein Alpha 12 (GNa12) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to G Protein Alpha 12 (GNa12) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for G Protein Alpha 12 (GNa12) CLIA Kit Customized Service Offer
n/a ELISA Kit for G Protein Alpha 12 (GNa12) ELISA Kit Customized Service Offer
  1. "Expression cDNA cloning of a transforming gene encoding the wild-type G alpha 12 gene product."Mol. Cell. Biol. 13:762-768(1993) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence of human chromosome 7." Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
  4. "Human chromosome 7: DNA sequence and biology." Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Socius, a novel binding partner of Galpha12/13, promotes the Galpha12-induced RhoA activation."Biochem. Biophys. Res. Commun. 337:615-620(2005) [PubMed] [Europe PMC] [Abstract]
  7. "RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits."Biol. Reprod. 79:1021-1029(2008) [PubMed] [Europe PMC] [Abstract]
  8. "PRR5L degradation promotes mTORC2-mediated PKC-delta phosphorylation and cell migration downstream of Galpha12."Nat. Cell Biol. 14:686-696(2012) [PubMed] [Europe PMC] [Abstract]