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Cell Division Cycle Protein 34 (CDC34)

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CDC34; E2-CDC34; UBC3; UBE2R1; Ubiquitin-Conjugating Enzyme E2 R1; Ubiquitin-protein ligase R1

Cell Division Cycle Protein 34 (CDC34)
CCC E611
HGNC 1734
MGI 102657
PubMed 10373550
RGD 1305411
UniProt P49427
Wiki CDC34
Ubiquitin-conjugating enzyme E2 R1 is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
In vitro binding experiments demonstrated that phosphorylated UBC3B and CDC34 bound specifically to the F-box protein beta-TRCP (BTRC), which resulted in enhanced degradation of beta-catenin, a substrate of BTRC. Semplici et al. (2002) suggested that CK2-dependent phosphorylation of CDC34 and UBC3B functions by regulating BTRC substrate recognition.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPE611Hu01 Recombinant Cell Division Cycle Protein 34 (CDC34) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Cell Division Cycle Protein 34 (CDC34) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cell Division Cycle Protein 34 (CDC34) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cell Division Cycle Protein 34 (CDC34) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cell Division Cycle Protein 34 (CDC34) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cell Division Cycle Protein 34 (CDC34) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cell Division Cycle Protein 34 (CDC34) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cell Division Cycle Protein 34 (CDC34) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cell Division Cycle Protein 34 (CDC34) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cell Division Cycle Protein 34 (CDC34) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cell Division Cycle Protein 34 (CDC34) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cell Division Cycle Protein 34 (CDC34) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cell Division Cycle Protein 34 (CDC34) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cell Division Cycle Protein 34 (CDC34) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cell Division Cycle Protein 34 (CDC34) ELISA Kit Customized Service Offer
  1. "Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast."Proc. Natl. Acad. Sci. U.S.A. 90:10484-10488(1993) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Identification of the ubiquitin carrier proteins, E2s, involved in signal-induced conjugation and subsequent degradation of IkappaBalpha."J. Biol. Chem. 274:14823-14830(1999) [PubMed] [Europe PMC] [Abstract]
  5. "Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis."Mol. Cell. Biol. 19:5001-5013(1999) [PubMed] [Europe PMC] [Abstract]
  6. "Association of human ubiquitin-conjugating enzyme CDC34 with the mitotic spindle in anaphase."J. Cell Sci. 113:1687-1694(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Degradation of B-Myb by ubiquitin-mediated proteolysis: involvement of the Cdc34-SCF(p45Skp2) pathway."Oncogene 19:2986-2995(2000) [PubMed] [Europe PMC] [Abstract]
  8. "SCF(beta-TRCP) and phosphorylation dependent ubiquitination of I kappa B alpha catalyzed by Ubc3 and Ubc4."Oncogene 19:3529-3536(2000) [PubMed] [Europe PMC] [Abstract]
  9. "Phosphorylation of the human ubiquitin-conjugating enzyme, CDC34, by casein kinase 2."J. Biol. Chem. 276:41049-41058(2001) [PubMed] [Europe PMC] [Abstract]
  10. "The infected cell protein 0 of herpes simplex virus 1 dynamically interacts with proteasomes, binds and activates the cdc34 E2 ubiquitin-conjugating enzyme, and possesses in vitro E3 ubiquitin ligase activity."Proc. Natl. Acad. Sci. U.S.A. 98:8815-8820(2001) [PubMed] [Europe PMC] [Abstract]
  11. "The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34."J. Biol. Chem. 277:516-527(2002) [PubMed] [Europe PMC] [Abstract]
  12. "CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation."Oncogene 21:3978-3987(2002) [PubMed] [Europe PMC] [Abstract]
  13. "Herpes simplex virus 1-infected cell protein 0 contains two E3 ubiquitin ligase sites specific for different E2 ubiquitin-conjugating enzymes."Proc. Natl. Acad. Sci. U.S.A. 99:631-636(2002) [PubMed] [Europe PMC] [Abstract]
  14. "Characterization of the novel E3 ubiquitin ligase encoded in exon 3 of herpes simplex virus-1-infected cell protein 0."Proc. Natl. Acad. Sci. U.S.A. 99:7889-7894(2002) [PubMed] [Europe PMC] [Abstract]
  15. "The human ubiquitin-conjugating enzyme Cdc34 controls cellular proliferation through regulation of p27Kip1 protein levels."Exp. Cell Res. 303:482-493(2005) [PubMed] [Europe PMC] [Abstract]
  16. "Cdc34 C-terminal tail phosphorylation regulates Skp1/cullin/F-box (SCF)-mediated ubiquitination and cell cycle progression."Biochem. J. 405:569-581(2007) [PubMed] [Europe PMC] [Abstract]
  17. "Human Cdc34 employs distinct sites to coordinate attachment of ubiquitin to a substrate and assembly of polyubiquitin chains."Mol. Cell. Biol. 27:7041-7052(2007) [PubMed] [Europe PMC] [Abstract]
  18. "Multimodal activation of the ubiquitin ligase SCF by Nedd8 conjugation."Mol. Cell 32:21-31(2008) [PubMed] [Europe PMC] [Abstract]
  19. "Rapid E2-E3 assembly and disassembly enable processive ubiquitylation of cullin-RING ubiquitin ligase substrates."Cell 139:957-968(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src."J. Biol. Chem. 284:5265-5276(2009) [PubMed] [Europe PMC] [Abstract]
  21. "let-7 Overexpression leads to an increased fraction of cells in G2/M, direct down-regulation of Cdc34, and stabilization of Wee1 kinase in primary fibroblasts."J. Biol. Chem. 284:6605-6609(2009) [PubMed] [Europe PMC] [Abstract]
  22. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
  23. "Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate."Mol. Cell 37:784-796(2010) [PubMed] [Europe PMC] [Abstract]
  24. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  25. "Human ubiquitin-conjugating enzyme cdc34."Submitted (FEB-2009) to the PDB data bank