Phospholipase C Eta 1 (PLCh1)

The deduced 1,002-amino acid PLCH1 isoform has a calculated molecular mass of 115 kD and shares similarity with other PLC family members. Northern blot analysis of human tissues detected a 7.4-kb transcript in brain and lung. Western blot analysis of mouse tissues detected Plch1 protein exclusively in nerve tissues such as cerebrum, cerebellum, and the spinal cord. In contrast to the mRNA data, Plch1 protein was not detected in lung. In situ hybridization detected mouse Plch1 mRNA expression throughout the brain with high expression in neuronal, cell-enriched regions. Fluorescence microscopy of HEK293 cells localized PLCH1 to the cytosol and membrane but not in the nucleus; in mouse brain tissues, Plch1 was detected in all cytosolic and membrane fractions.