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ATP Dependent DNA ligase IV (LIG4)

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Polydeoxyribonucleotide Synthase[ATP]4; Polynucleotide Ligase; Sealase; DNA Repair Enzyme; DNA Joinase; Polydeoxyribonucleotide synthase [ATP] 4

ATP Dependent DNA ligase IV (LIG4)
CCC D630
HGNC 6601
MGI 1335098
PubMed 18165945
RGD 1304639
UniProt P49917
Wiki LIG4
LIG4 is an ATP-dependent DNA ligase that joins double-strand breaks during the non-homologous end joining pathway of double-strand break repair. It is also essential for V(D)J recombination. Lig4 forms a complex with XRCC4, and further interacts with the DNA-dependent protein kinase (DNA-PK) and XLF/Cernunnos, which are also required for NHEJ. The crystal structure of the Lig4/XRCC4 complex has been resolved.
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ATP Dependent DNA ligase IV (LIG4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ATP Dependent DNA ligase IV (LIG4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ATP Dependent DNA ligase IV (LIG4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ATP Dependent DNA ligase IV (LIG4) CLIA Kit Customized Service Offer
n/a ELISA Kit for ATP Dependent DNA ligase IV (LIG4) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ATP Dependent DNA ligase IV (LIG4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ATP Dependent DNA ligase IV (LIG4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ATP Dependent DNA ligase IV (LIG4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ATP Dependent DNA ligase IV (LIG4) CLIA Kit Customized Service Offer
n/a ELISA Kit for ATP Dependent DNA ligase IV (LIG4) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ATP Dependent DNA ligase IV (LIG4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ATP Dependent DNA ligase IV (LIG4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ATP Dependent DNA ligase IV (LIG4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ATP Dependent DNA ligase IV (LIG4) CLIA Kit Customized Service Offer
n/a ELISA Kit for ATP Dependent DNA ligase IV (LIG4) ELISA Kit Customized Service Offer
  1. "Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination."Mol. Cell. Biol. 15:3206-3216(1995) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and analysis of human chromosome 13." Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "DNA ligase IV from HeLa cell nuclei."J. Biol. Chem. 271:24257-24261(1996) [PubMed] [Europe PMC] [Abstract]
  5. "DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes."Mol. Cell 2:477-484(1998) [PubMed] [Europe PMC] [Abstract]
  6. "Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV."Curr. Biol. 7:588-598(1997) [PubMed] [Europe PMC] [Abstract]
  7. "Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase."J. Biol. Chem. 275:26196-26205(2000) [PubMed] [Europe PMC] [Abstract]
  8. "Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment."J. Mol. Biol. 326:93-103(2003) [PubMed] [Europe PMC] [Abstract]
  9. "A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses."EMBO J. 26:2094-2103(2007) [PubMed] [Europe PMC] [Abstract]
  10. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  11. "Crystal structure of an Xrcc4-DNA ligase IV complex."Nat. Struct. Biol. 8:1015-1019(2001) [PubMed] [Europe PMC] [Abstract]
  12. "Solution structure of the first BRCT domain of human DNA ligase IV."Submitted (DEC-2006) to the PDB data bank
  13. "Identification of a defect in DNA ligase IV in a radiosensitive leukaemia patient."Curr. Biol. 9:699-702(1999) [PubMed] [Europe PMC] [Abstract]
  14. "Cellular and biochemical impact of a mutation in DNA ligase IV conferring clinical radiosensitivity."J. Biol. Chem. 276:31124-31132(2001) [PubMed] [Europe PMC] [Abstract]
  15. "DNA ligase IV mutations identified in patients exhibiting developmental delay and immunodeficiency."Mol. Cell 8:1175-1185(2001) [PubMed] [Europe PMC] [Abstract]
  16. "Genetic variants of NHEJ DNA ligase IV can affect the risk of developing multiple myeloma, a tumour characterised by aberrant class switch recombination."J. Med. Genet. 39:900-905(2002) [PubMed] [Europe PMC] [Abstract]
  17. "A new type of radiosensitive T-B-NK(+) severe combined immunodeficiency caused by a LIG4 mutation."J. Clin. Invest. 116:137-145(2006) [PubMed] [Europe PMC] [Abstract]