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6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3)

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IPFK2; PFK2; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56

6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3)
CCC D401
HGNC 8874
MGI 2181202
PubMed 19473963
RGD 619776
UniProt Q16875
Wiki PFKFB3
The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase(PFKFB) regulates the steady-state concentration of fructose-2,6-bisphosphate, a potent activator of a key regulatory enzyme of glycolysis, phosphofructokinase.The predicted PFKFB3 protein, which is 61% similar to human liver PFKFB, contains 529 amino acids and 7 potential phosphorylation sites. Northern blot analysis of first-trimester and term placentas detected a 4.5-kb PFKFB3 transcript.
PFKFB3 is expressed as a 58-kD protein. Molecular sieve analysis confirmed that PFKFB3 is bifunctional, although the phosphatase activity was low due to a mutation in an active site. The kinase activity was upregulated after phosphorylation by protein kinases.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) CLIA Kit Customized Service Offer
n/a ELISA Kit for 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) CLIA Kit Customized Service Offer
n/a ELISA Kit for 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) CLIA Kit Customized Service Offer
n/a ELISA Kit for 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 (PFKFB3) ELISA Kit Customized Service Offer
  1. "Cloning of cDNA encoding for a novel isozyme of fructose 6-phosphate, 2-kinase/fructose 2,6-bisphosphatase from human placenta."J. Biochem. 119:506-511(1996) [PubMed] [Europe PMC] [Abstract]
  2. "The third human isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3) map position 10p14-p15."Chromosome Res. 5:150-150(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Molecular cloning, expression, and chromosomal localization of a ubiquitously expressed human 6-phosphofructo-2-kinase/ fructose-2, 6-bisphosphatase gene (PFKFB3)."Cytogenet. Cell Genet. 83:214-217(1998) [PubMed] [Europe PMC] [Abstract]
  4. "An inducible gene product for 6-phosphofructo-2-kinase with an AU-rich instability element: role in tumor cell glycolysis and the Warburg effect."Proc. Natl. Acad. Sci. U.S.A. 96:3047-3052(1999) [PubMed] [Europe PMC] [Abstract]
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Antigens recognized by autologous antibody in patients with renal-cell carcinoma."Int. J. Cancer 83:456-464(1999) [PubMed] [Europe PMC] [Abstract]
  9. "The stimulation of glycolysis by hypoxia in activated monocytes is mediated by AMP-activated protein kinase and inducible 6-phosphofructo-2-kinase."J. Biol. Chem. 277:30778-30783(2002) [PubMed] [Europe PMC] [Abstract]
  10. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy."J. Biol. Chem. 281:2939-2944(2006) [PubMed] [Europe PMC] [Abstract]
  14. "A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase."J. Mol. Biol. 370:14-26(2007) [PubMed] [Europe PMC] [Abstract]
  15. "Molecular basis of the fructose-2,6-bisphosphatase reaction of PFKFB3: transition state and the C-terminal function."Proteins 80:1143-1153(2012) [PubMed] [Europe PMC] [Abstract]