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Insulin Receptor Substrate 1 (IRS1)

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HIRS1

Insulin Receptor Substrate 1 (IRS1)
CCC C546
HGNC 6125
MGI 99454
PubMed 20074531
RGD 2922
UniProt P35568
Wiki IRS1
Insulin receptor substrate 1 plays a key role in transmitting signals from the IGF-1 receptors to intracellular pathways PI3K / Akt and Erk MAP kinase pathways. Tyrosine phosphorylation of the insulin receptors or IGF-1 receptors, upon extracellular ligand binding, induces the cytoplasmic binding of IRS-1 to these receptors, through its SH2 domains.
IRS-1 plays important biological function for both metabolic and mitogenic (growth promoting) pathways: mice deficent of IRS1 have only a mild diabetic phenotype, but a pronounced growth impairment, i.e. IRS-1 knockout mice only reach 50% of the weight of normal mice. IRS-1 may also play a role in cancer, as it has been shown that transgenic mice overexpressing IRS-1 develop breast cancer.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC546Hu01 Recombinant Insulin Receptor Substrate 1 (IRS1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC546Hu01 Polyclonal Antibody to Insulin Receptor Substrate 1 (IRS1) WB; IHC; ICC; IP.
MAC546Hu21 Monoclonal Antibody to Insulin Receptor Substrate 1 (IRS1) WB; IHC; ICC; IP.
Assay Kits SEC546Hu ELISA Kit for Insulin Receptor Substrate 1 (IRS1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC546Mu01 Recombinant Insulin Receptor Substrate 1 (IRS1) Positive Control; Immunogen; SDS-PAGE; WB.
RPC546Mu02 Recombinant Insulin Receptor Substrate 1 (IRS1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC546Mu01 Polyclonal Antibody to Insulin Receptor Substrate 1 (IRS1) WB; IHC; ICC; IP.
PAC546Mu02 Polyclonal Antibody to Insulin Receptor Substrate 1 (IRS1) WB; IHC; ICC; IP.
LAC546Mu81 FITC-Linked Polyclonal Antibody to Insulin Receptor Substrate 1 (IRS1) WB; IHC; ICC; IF.
Assay Kits SEC546Mu ELISA Kit for Insulin Receptor Substrate 1 (IRS1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Insulin Receptor Substrate 1 (IRS1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Insulin Receptor Substrate 1 (IRS1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Insulin Receptor Substrate 1 (IRS1) Polyclonal Antibody Customized Service Offer
Assay Kits SEC546Ra ELISA Kit for Insulin Receptor Substrate 1 (IRS1) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Human skeletal muscle insulin receptor substrate-1. Characterization of the cDNA, gene, and chromosomal localization."Diabetes 42:1041-1054(1993) [PubMed] [Europe PMC] [Abstract]
  2. "Cloning and increased expression of an insulin receptor substrate-1-like gene in human hepatocellular carcinoma."Biochem. Biophys. Res. Commun. 183:280-285(1992) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The insulin receptor substrate (IRS-1) is a PEST protein that is susceptible to calpain degradation in vitro."Biochem. Biophys. Res. Commun. 196:767-772(1993) [PubMed] [Europe PMC] [Abstract]
  5. "Non-SH2 domains within insulin receptor substrate-1 and SHC mediate their phosphotyrosine-dependent interaction with the NPEY motif of the insulin-like growth factor I receptor."J. Biol. Chem. 270:15639-15643(1995) [PubMed] [Europe PMC] [Abstract]
  6. "Distinct modes of interaction of SHC and insulin receptor substrate-1 with the insulin receptor NPEY region via non-SH2 domains."J. Biol. Chem. 270:23258-23262(1995) [PubMed] [Europe PMC] [Abstract]
  7. "Phosphotyrosine-dependent interaction of SHC and insulin receptor substrate 1 with the NPEY motif of the insulin receptor via a novel non-SH2 domain."Mol. Cell. Biol. 15:2500-2508(1995) [PubMed] [Europe PMC] [Abstract]
  8. "Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2."J. Biol. Chem. 278:18440-18447(2003) [PubMed] [Europe PMC] [Abstract]
  9. "Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101)."J. Biol. Chem. 279:45304-45307(2004) [PubMed] [Europe PMC] [Abstract]
  10. "ALK receptor tyrosine kinase promotes cell growth and neurite outgrowth."J. Cell Sci. 117:3319-3329(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Deletion of SOCS7 leads to enhanced insulin action and enlarged islets of Langerhans."J. Clin. Invest. 115:2462-2471(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
  13. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  14. "Recruitment of insulin receptor substrate-1 and activation of NF-kappaB essential for midkine growth signaling through anaplastic lymphoma kinase."Oncogene 26:859-869(2007) [PubMed] [Europe PMC] [Abstract]
  15. "S6K directly phosphorylates IRS-1 on Ser-270 to promote insulin resistance in response to TNF-(alpha) signaling through IKK2."J. Biol. Chem. 283:35375-35382(2008) [PubMed] [Europe PMC] [Abstract]
  16. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  17. "The CUL7 E3 ubiquitin ligase targets insulin receptor substrate 1 for ubiquitin-dependent degradation."Mol. Cell 30:403-414(2008) [PubMed] [Europe PMC] [Abstract]
  18. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  20. "The double-stranded RNA-dependent protein kinase differentially regulates insulin receptor substrates 1 and 2 in HepG2 cells."Mol. Biol. Cell 21:3449-3458(2010) [PubMed] [Europe PMC] [Abstract]
  21. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  22. "Structural basis for IL-4 receptor phosphopeptide recognition by the IRS-1 PTB domain."Nat. Struct. Biol. 3:388-393(1996) [PubMed] [Europe PMC] [Abstract]
  23. "Crystal structure of the pleckstrin homology-phosphotyrosine binding (PH-PTB) targeting region of insulin receptor substrate 1."Proc. Natl. Acad. Sci. U.S.A. 96:8378-8383(1999) [PubMed] [Europe PMC] [Abstract]
  24. "Structure and autoregulation of the insulin-like growth factor 1 receptor kinase."Nat. Struct. Biol. 8:1058-1063(2001) [PubMed] [Europe PMC] [Abstract]
  25. "Aminoacid polymorphisms of insulin receptor substrate-1 in non-insulin-dependent diabetes mellitus."Lancet 342:828-832(1993) [PubMed] [Europe PMC] [Abstract]
  26. "Deletion of Gly723 in the insulin receptor substrate-1 of a patient with noninsulin-dependent diabetes mellitus."Hum. Mutat. 7:364-366(1996) [PubMed] [Europe PMC] [Abstract]
  27. "Novel allele of the insulin receptor substrate-1 bearing two non-conservative amino acid substitutions in a patient with noninsulin-dependent diabetes mellitus."Hum. Mutat. 11:411-411(1998) [PubMed] [Europe PMC] [Abstract]
  28. "The Gly-->Arg(972) amino acid polymorphism in insulin receptor substrate-1 affects glucose metabolism in skeletal muscle cells."J. Clin. Endocrinol. Metab. 85:2004-2013(2000) [PubMed] [Europe PMC] [Abstract]
  29. "The Arg(972) variant in insulin receptor substrate-1 is associated with an atherogenic profile in offspring of type 2 diabetic patients."J. Clin. Endocrinol. Metab. 88:3368-3371(2003) [PubMed] [Europe PMC] [Abstract]
  30. "Genetic polymorphism PC-1 K121Q and ethnic susceptibility to insulin resistance."J. Clin. Endocrinol. Metab. 88:5927-5934(2003) [PubMed] [Europe PMC] [Abstract]
  31. "A novel T608R missense mutation in insulin receptor substrate-1 identified in a subject with type 2 diabetes impairs metabolic insulin signaling."J. Clin. Endocrinol. Metab. 88:1468-1475(2003) [PubMed] [Europe PMC] [Abstract]
  32. "G972R IRS-1 variant impairs insulin regulation of endothelial nitric oxide synthase in cultured human endothelial cells."Circulation 109:399-405(2004) [PubMed] [Europe PMC] [Abstract]
  33. "Human insulin receptor substrate-1 (IRS-1) polymorphism G972R causes IRS-1 to associate with the insulin receptor and inhibit receptor autophosphorylation."J. Biol. Chem. 280:6441-6446(2005) [PubMed] [Europe PMC] [Abstract]