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Prostate-specific Membrane Antigen (PMSA)

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FOLH1; PSM; FGCP; FOLH; GCP2; NAALAD1; GCPII; mGCP; NAALADase I; Glutamate Carboxypeptidase II; Folate Hydrolase 1; Folylpoly-gamma-glutamate carboxypeptidase

Prostate-specific Membrane Antigen (PMSA)
CCC C512
HGNC 3788
MGI 1858193
PubMed 17684227
RGD 70963
UniProt Q04609
Wiki GCPII

Glutamate carboxypeptidase II is a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC512Hu01 Recombinant Prostate-specific Membrane Antigen (PMSA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC512Hu01 Polyclonal Antibody to Prostate-specific Membrane Antigen (PMSA) WB; IHC
LAC512Hu71 Biotin-Linked Polyclonal Antibody to Prostate-specific Membrane Antigen (PMSA) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Prostate-specific Membrane Antigen (PMSA) CLIA Kit Customized Service Offer
n/a ELISA Kit for Prostate-specific Membrane Antigen (PMSA) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC512Mu01 Recombinant Prostate-specific Membrane Antigen (PMSA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC512Mu01 Polyclonal Antibody to Prostate-specific Membrane Antigen (PMSA) WB; IHC
Assay Kits n/a CLIA Kit for Prostate-specific Membrane Antigen (PMSA) CLIA Kit Customized Service Offer
n/a ELISA Kit for Prostate-specific Membrane Antigen (PMSA) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Prostate-specific Membrane Antigen (PMSA) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Prostate-specific Membrane Antigen (PMSA) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Prostate-specific Membrane Antigen (PMSA) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Prostate-specific Membrane Antigen (PMSA) CLIA Kit Customized Service Offer
n/a ELISA Kit for Prostate-specific Membrane Antigen (PMSA) ELISA Kit Customized Service Offer

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC512Po01 Recombinant Prostate-specific Membrane Antigen (PMSA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC512Po01 Polyclonal Antibody to Prostate-specific Membrane Antigen (PMSA) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Prostate-specific Membrane Antigen (PMSA) CLIA Kit Customized Service Offer
n/a ELISA Kit for Prostate-specific Membrane Antigen (PMSA) ELISA Kit Customized Service Offer
  1. "Molecular cloning of a complementary DNA encoding a prostate-specific membrane antigen."Cancer Res. 53:227-230(1993) [PubMed] [Europe PMC] [Abstract]
  2. "Alternatively spliced variants of prostate-specific membrane antigen RNA: ratio of expression as a potential measurement of progression."Cancer Res. 55:1441-1443(1995) [PubMed] [Europe PMC] [Abstract]
  3. "Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene."Biochim. Biophys. Acta 1443:113-127(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Molecular characterization of human brain N-acetylated alpha-linked acidic dipeptidase (NAALADase)."J. Pharmacol. Exp. Ther. 286:1020-1025(1998) [PubMed] [Europe PMC] [Abstract]
  5. "Isolation and expression of novel human glutamate carboxypeptidases with N-acetylated alpha-linked acidic dipeptidase and dipeptidyl peptidase IV activity."J. Biol. Chem. 274:8470-8483(1999) [PubMed] [Europe PMC] [Abstract]
  6. "Glutamate carboxypeptidase II: a polymorphism associated with lower levels of serum folate and hyperhomocysteinemia."Hum. Mol. Genet. 9:2837-2844(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Cloning and sequencing of Chinese prostate-specific membrane antigen."Mian Yi Xue Za Zhi 17:328-330(2001)
  8. "High expression of PSM-E correlated with tumor grade in prostate cancer: a new alternatively spliced variant of prostate-specific membrane antigen."Prostate 67:1791-1800(2007) [PubMed] [Europe PMC] [Abstract]
  9. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Human chromosome 11 DNA sequence and analysis including novel gene identification." Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
  11. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  12. "Molecular cloning of a peptidase against N-acetylaspartylglutamate from a rat hippocampal cDNA library."J. Neurochem. 69:2270-2277(1997) [PubMed] [Europe PMC] [Abstract]
  13. "Identification, purification, and subcellular localization of prostate-specific membrane antigen PSM' protein in the LNCaP prostatic carcinoma cell line."Cancer Res. 58:4787-4789(1998) [PubMed] [Europe PMC] [Abstract]
  14. "Molecular cloning of alternatively spliced variants of the peptidase against N-acetylaspartylglutamate (NAAG) from human and rat nervous systems."Abstr. - Soc. Neurosci. 24:579-579(1998)
  15. "Hydrolysis of the neuropeptide N-acetylaspartylglutamate (NAAG) by cloned human glutamate carboxypeptidase II."Brain Res. 795:341-348(1998) [PubMed] [Europe PMC] [Abstract]
  16. "Structure of membrane glutamate carboxypeptidase."Biochim. Biophys. Acta 1339:247-252(1997) [PubMed] [Europe PMC] [Abstract]
  17. "Site-directed mutagenesis of predicted active site residues in glutamate carboxypeptidase II."Mol. Pharmacol. 55:179-185(1999) [PubMed] [Europe PMC] [Abstract]
  18. "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
  19. "Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity."Protein Sci. 13:1627-1635(2004) [PubMed] [Europe PMC] [Abstract]
  20. "Comparative analysis of prostate-specific membrane antigen (PSMA) versus a prostate-specific membrane antigen-like gene."Prostate 58:200-210(2004) [PubMed] [Europe PMC] [Abstract]
  21. "Expression of prostate-specific membrane antigen in normal and malignant human tissues."World J. Surg. 30:628-636(2006) [PubMed] [Europe PMC] [Abstract]
  22. "Expression of glutamate carboxypeptidase II in human brain."Neuroscience 144:1361-1372(2007) [PubMed] [Europe PMC] [Abstract]
  23. "Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer."EMBO J. 25:1375-1384(2006) [PubMed] [Europe PMC] [Abstract]
  24. "Human glutamate carboxypeptidase II inhibition: structures of GCPII in complex with two potent inhibitors, quisqualate and 2-PMPA."Acta Crystallogr. D 63:508-513(2007) [PubMed] [Europe PMC] [Abstract]
  25. "Structural insight into the pharmacophore pocket of human glutamate carboxypeptidase II."J. Med. Chem. 50:3267-3273(2007) [PubMed] [Europe PMC] [Abstract]
  26. "Interactions between human glutamate carboxypeptidase II and urea-based inhibitors: structural characterization."J. Med. Chem. 51:7737-7743(2008) [PubMed] [Europe PMC] [Abstract]
  27. "Structural basis of interactions between human glutamate carboxypeptidase II and its substrate analogs."J. Mol. Biol. 376:1438-1450(2008) [PubMed] [Europe PMC] [Abstract]
  28. "Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography, and computational methods."Biochemistry 48:4126-4138(2009) [PubMed] [Europe PMC] [Abstract]
  29. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]