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Mediator Complex Subunit 1 (MED1)

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PPARBP; ARC205; PBP; CRSP1; CRSP200; PPARGBP; RB18A; TRAP220; TRIP2; Peroxisome proliferator-activated receptor-binding protein; Thyroid receptor-interacting protein 2

Mediator Complex Subunit 1 (MED1)
CCC C238
HGNC 9234
MGI 1100846
PubMed 21480322
RGD 1559552
UniProt Q15648
Wiki MED1

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. Mediator of RNA polymerase II transcription subunit 1 is protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC238Hu01 Recombinant Mediator Complex Subunit 1 (MED1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC238Hu01 Polyclonal Antibody to Mediator Complex Subunit 1 (MED1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Mediator Complex Subunit 1 (MED1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mediator Complex Subunit 1 (MED1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mediator Complex Subunit 1 (MED1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mediator Complex Subunit 1 (MED1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mediator Complex Subunit 1 (MED1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mediator Complex Subunit 1 (MED1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mediator Complex Subunit 1 (MED1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mediator Complex Subunit 1 (MED1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mediator Complex Subunit 1 (MED1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mediator Complex Subunit 1 (MED1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mediator Complex Subunit 1 (MED1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mediator Complex Subunit 1 (MED1) ELISA Kit Customized Service Offer
  1. "Identification of RB18A, a 205 kDa new p53 regulatory protein which shares antigenic and functional properties with p53."Oncogene 15:3013-3024(1997) [PubMed] [Europe PMC] [Abstract]
  2. "The TRAP220 component of a thyroid hormone receptor-associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion."Proc. Natl. Acad. Sci. U.S.A. 95:7939-7944(1998) [PubMed] [Europe PMC] [Abstract]
  3. ErratumProc. Natl. Acad. Sci. U.S.A. 95:14584-14584(1998)
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Composite co-activator ARC mediates chromatin-directed transcriptional activation."Nature 398:828-832(1999) [PubMed] [Europe PMC] [Abstract]
  6. "The DRIP complex and SRC-1/p160 coactivators share similar nuclear receptor binding determinants but constitute functionally distinct complexes."Mol. Cell. Biol. 20:2718-2726(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex."Nature 398:824-828(1999) [PubMed] [Europe PMC] [Abstract]
  8. "Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor."Mol. Endocrinol. 9:243-254(1995) [PubMed] [Europe PMC] [Abstract]
  9. "A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation."Mol. Cell 3:97-108(1999) [PubMed] [Europe PMC] [Abstract]
  10. ErratumMol. Cell 3:541-541(1999)
  11. "Identification of mouse TRAP100: a transcriptional coregulatory factor for thyroid hormone and vitamin D receptors."Mol. Endocrinol. 13:1130-1140(1999) [PubMed] [Europe PMC] [Abstract]
  12. "Coactivators for the orphan nuclear receptor RORalpha."Mol. Endocrinol. 13:1550-1557(1999) [PubMed] [Europe PMC] [Abstract]
  13. "Functional interactions between the estrogen receptor and DRIP205, a subunit of the heteromeric DRIP coactivator complex."J. Biol. Chem. 275:20928-20934(2000) [PubMed] [Europe PMC] [Abstract]
  14. "Differential recruitment of the mammalian mediator subunit TRAP220 by estrogen receptors ERalpha and ERbeta."J. Biol. Chem. 276:23397-23404(2001) [PubMed] [Europe PMC] [Abstract]
  15. "A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression."J. Biol. Chem. 277:42852-42858(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis."Nature 417:563-567(2002) [PubMed] [Europe PMC] [Abstract]
  17. "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro."Proc. Natl. Acad. Sci. U.S.A. 99:2642-2647(2002) [PubMed] [Europe PMC] [Abstract]
  18. "Ordered recruitment of histone acetyltransferases and the TRAP/Mediator complex to thyroid hormone-responsive promoters in vivo."Proc. Natl. Acad. Sci. U.S.A. 99:7934-7939(2002) [PubMed] [Europe PMC] [Abstract]
  19. "An extended LXXLL motif sequence determines the nuclear receptor binding specificity of TRAP220."J. Biol. Chem. 278:10942-10951(2003) [PubMed] [Europe PMC] [Abstract]
  20. "Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1alpha."Mol. Cell 12:1137-1149(2003) [PubMed] [Europe PMC] [Abstract]
  21. "Vitamin D-interacting protein 205 (DRIP205) coactivation of estrogen receptor alpha (ERalpha) involves multiple domains of both proteins."J. Biol. Chem. 279:53602-53612(2004) [PubMed] [Europe PMC] [Abstract]
  22. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
  23. "Structural and functional organization of TRAP220, the TRAP/mediator subunit that is targeted by nuclear receptors."Mol. Cell. Biol. 24:8244-8254(2004) [PubMed] [Europe PMC] [Abstract]
  24. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
  25. "Activation of TRAP/mediator subunit TRAP220/Med1 is regulated by mitogen-activated protein kinase-dependent phosphorylation."Mol. Cell. Biol. 25:10695-10710(2005) [PubMed] [Europe PMC] [Abstract]
  26. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  27. "Regulation of Aurora-A kinase gene expression via GABP recruitment of TRAP220/MED1."J. Biol. Chem. 281:14691-14699(2006) [PubMed] [Europe PMC] [Abstract]
  28. "Mediator modulates Gli3-dependent Sonic hedgehog signaling."Mol. Cell. Biol. 26:8667-8682(2006) [PubMed] [Europe PMC] [Abstract]
  29. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  30. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  31. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  32. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  33. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  34. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  35. "The basic helix-loop-helix proteins differentiated embryo chondrocyte (DEC) 1 and DEC2 function as corepressors of retinoid X receptors."Mol. Pharmacol. 76:1360-1369(2009) [PubMed] [Europe PMC] [Abstract]
  36. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  37. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  38. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  39. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  40. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  41. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]