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Phospholipase D (PLD)

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PLD1; Choline Phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1

Phospholipase D (PLD)
CCC C049
HGNC 9067
MGI 109585
PubMed 15052340
RGD 3349
UniProt Q13393
Wiki PLD

Phospholipase D is an enzyme which is located in the plasma membrane and catalyzes the hydrolysis of phosphatidylcholine to form phosphatidic acid (PA), releasing the soluble choline headgroup into the cytosol. There are two mammalian isoforms of phospholipase D: PLD1 and PLD2. PLD directly interacts with kinases like PKC, ERK, TYK and controls the signalling indicating that PLD is activated by these kinases.

As choline is very abundant in the cell, PLD activity does not significantly affect choline levels, and choline is unlikely to play any role in signalling.  Phosphatidic acid is a signal molecule and acts to recruit SK1 to membranes. PA is extremely short lived and is rapidly hydrolysed by the enzyme PA phosphohydrolase to form diacylglycerol (DAG).

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC049Hu01 Recombinant Phospholipase D (PLD) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC049Hu01 Polyclonal Antibody to Phospholipase D (PLD) WB; IHC; ICC; IP.
MAC049Hu22 Monoclonal Antibody to Phospholipase D (PLD) WB; IHC; ICC; IP.
Assay Kits SEC049Hu ELISA Kit for Phospholipase D (PLD) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC049Hu CLIA Kit for Phospholipase D (PLD) Chemiluminescent immunoassay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phospholipase D (PLD) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phospholipase D (PLD) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phospholipase D (PLD) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phospholipase D (PLD) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phospholipase D (PLD) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phospholipase D (PLD) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phospholipase D (PLD) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phospholipase D (PLD) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phospholipase D (PLD) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phospholipase D (PLD) ELISA Kit Customized Service Offer
  1. "Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family."J. Biol. Chem. 270:29640-29643(1995) [PubMed] [Europe PMC] [Abstract]
  2. "Characterization of two alternately spliced forms of phospholipase D1. Activation of the purified enzymes by phosphatidylinositol 4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric GTP-binding proteins and protein kinase C-alpha."J. Biol. Chem. 272:3860-3868(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Characterization of human PLD2 and the analysis of PLD isoform splice variants."FASEB J. 12:1309-1317(1998) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Cloning and initial characterization of a human phospholipase D2 (hPLD2). ADP-ribosylation factor regulates hPLD2."J. Biol. Chem. 273:12846-12852(1998) [PubMed] [Europe PMC] [Abstract]
  6. "Interaction of the type Ialpha PIPkinase with phospholipase D: a role for the local generation of phosphatidylinositol 4, 5-bisphosphate in the regulation of PLD2 activity."EMBO J. 19:5440-5449(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]