≡

Eukaryotic Translation Initiation Factor 3A (EIF3A)

[Edit]

EIF3S10; EIF3-A; EIF3; P167; EIF3-P170; EIF3-Theta; P180; P185; Eukaryotic translation initiation factor 3 subunit 10

Eukaryotic Translation Initiation Factor 3A (EIF3A)
CCC B885
HGNC 3271
MGI 95301
PubMed 20647036
UniProt Q14152
Wiki EIF3A

EIF3, at 650 kD, is the largest of the EIFs. According to Johnson et al. (1997), EIF3 has been implicated in several roles, including binding to the 40S ribosomal subunit and to other EIFs, possibly to align the factors for initial binding to the 40S subunit and the subsequent identification of the AUG initiation codon. The EIF3 protein synthesis initiation factor is composed of at least 8 subunits, the largest of which is p180.Using a library of endoribonuclease-prepared short interfering RNAs (esiRNAs), Kittler et al. (2004) identified 37 genes required for cell division, one of which was EIF3S10. These 37 genes included several splicing factors for which knockdown generates mitotic spindle defects. In addition, a putative nuclear-export terminator was found to speed up cell proliferation and mitotic progression after knockdown.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Eukaryotic Translation Initiation Factor 3A (EIF3A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Eukaryotic Translation Initiation Factor 3A (EIF3A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Eukaryotic Translation Initiation Factor 3A (EIF3A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Eukaryotic Translation Initiation Factor 3A (EIF3A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Eukaryotic Translation Initiation Factor 3A (EIF3A) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB885Mu01 Recombinant Eukaryotic Translation Initiation Factor 3A (EIF3A) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB885Mu01 Polyclonal Antibody to Eukaryotic Translation Initiation Factor 3A (EIF3A) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Eukaryotic Translation Initiation Factor 3A (EIF3A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Eukaryotic Translation Initiation Factor 3A (EIF3A) ELISA Kit Customized Service Offer
  1. "The human p167 gene encodes a unique structural protein that contains centrosomin A homology and associates with a multicomponent complex."DNA Cell Biol. 16:515-531(1997) [PubMed] [Europe PMC] [Abstract]
  2. "Identification of cDNA clones for the large subunit of eukaryotic translation initiation factor 3. Comparison of homologues from human, Nicotiana tabacum, Caenorhabditis elegans, and Saccharomyces cerevisiae."J. Biol. Chem. 272:7106-7113(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1."DNA Res. 2:167-174(1995) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
  6. , Submitted (JUL-2007) to UniProtKB
  7. "Molecular interaction between human tumor marker protein p150, the largest subunit of eIF3, and intermediate filament protein K7."J. Cell. Biochem. 80:483-490(2001) [PubMed] [Europe PMC] [Abstract]
  8. "Characterization of eIF3k: a newly discovered subunit of mammalian translation initiation factor eIF3."Eur. J. Biochem. 270:4133-4139(2003) [PubMed] [Europe PMC] [Abstract]
  9. "The j-subunit of human translation initiation factor eIF3 is required for the stable binding of eIF3 and its subcomplexes to 40 S ribosomal subunits in vitro."J. Biol. Chem. 279:8946-8956(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association."RNA 11:470-486(2005) [PubMed] [Europe PMC] [Abstract]
  11. "Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit."J. Biol. Chem. 281:22917-22932(2006) [PubMed] [Europe PMC] [Abstract]
  12. "Reconstitution reveals the functional core of mammalian eIF3."EMBO J. 26:3373-3383(2007) [PubMed] [Europe PMC] [Abstract]
  13. "Human INT6/eIF3e is required for nonsense-mediated mRNA decay."EMBO Rep. 8:596-602(2007) [PubMed] [Europe PMC] [Abstract]
  14. "Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry."Mol. Cell. Proteomics 6:1135-1146(2007) [PubMed] [Europe PMC] [Abstract]
  15. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  16. "Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3."Proc. Natl. Acad. Sci. U.S.A. 105:18139-18144(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  20. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  21. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  22. "Structural roles for human translation factor eIF3 in initiation of protein synthesis."Science 310:1513-1515(2005) [PubMed] [Europe PMC] [Abstract]