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Protein Tyrosine Kinase 2 Beta (PYK2)

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PKB; CADTK; CAKB; FADK2; FAK2; PTK; PTK2b; RAFTK; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; Focal adhesion kinase 2; Proline-rich tyrosine kinase 2

Protein Tyrosine Kinase 2 Beta (PYK2)
CCC B322
HGNC 9612
MGI 104908
PubMed 20737507
RGD 628758
UniProt Q14289
Wiki PTK2B

Protein tyrosine kinase 2 beta is a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Tyrosine Kinase 2 Beta (PYK2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Tyrosine Kinase 2 Beta (PYK2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Tyrosine Kinase 2 Beta (PYK2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Tyrosine Kinase 2 Beta (PYK2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Tyrosine Kinase 2 Beta (PYK2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB322Mu01 Recombinant Protein Tyrosine Kinase 2 Beta (PYK2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB322Mu01 Polyclonal Antibody to Protein Tyrosine Kinase 2 Beta (PYK2) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Protein Tyrosine Kinase 2 Beta (PYK2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Tyrosine Kinase 2 Beta (PYK2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Tyrosine Kinase 2 Beta (PYK2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Tyrosine Kinase 2 Beta (PYK2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Tyrosine Kinase 2 Beta (PYK2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Tyrosine Kinase 2 Beta (PYK2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Tyrosine Kinase 2 Beta (PYK2) ELISA Kit Customized Service Offer
  1. "Protein tyrosine kinase PYK2 involved in Ca(2+)-induced regulation of ion channel and MAP kinase functions."Nature 376:737-745(1995) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning and assignment of FAK2, a novel human focal adhesion kinase, to 8p11.2-p22 by nonisotopic in situ hybridization."Genomics 32:484-486(1996) [PubMed] [Europe PMC] [Abstract]
  3. "Cloning and characterization of cell adhesion kinase beta, a novel protein-tyrosine kinase of the focal adhesion kinase subfamily."J. Biol. Chem. 270:21206-21219(1995) [PubMed] [Europe PMC] [Abstract]
  4. "Identification and characterization of a novel related adhesion focal tyrosine kinase (RAFTK) from megakaryocytes and brain."J. Biol. Chem. 270:27742-27751(1995) [PubMed] [Europe PMC] [Abstract]
  5. "A calcium-dependent tyrosine kinase splice variant in human monocytes. Activation by a two-stage process involving adherence and a subsequent intracellular signal."J. Biol. Chem. 273:9361-9364(1998) [PubMed] [Europe PMC] [Abstract]
  6. "DNA sequence and analysis of human chromosome 8." Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "A role for Pyk2 and Src in linking G-protein-coupled receptors with MAP kinase activation."Nature 383:547-550(1996) [PubMed] [Europe PMC] [Abstract]
  9. "Activation of Pyk2 by stress signals and coupling with JNK signaling pathway."Science 273:792-794(1996) [PubMed] [Europe PMC] [Abstract]
  10. "Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions."J. Biol. Chem. 273:1003-1014(1998) [PubMed] [Europe PMC] [Abstract]
  11. "Identification of a new Pyk2 target protein with Arf-GAP activity."Mol. Cell. Biol. 19:2338-2350(1999) [PubMed] [Europe PMC] [Abstract]
  12. "Suppression of Pyk2 kinase and cellular activities by FIP200."J. Cell Biol. 149:423-430(2000) [PubMed] [Europe PMC] [Abstract]
  13. "Nephrocystin interacts with Pyk2, p130(Cas), and tensin and triggers phosphorylation of Pyk2."Proc. Natl. Acad. Sci. U.S.A. 98:9784-9789(2001) [PubMed] [Europe PMC] [Abstract]
  14. "The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1."J. Biol. Chem. 278:29560-29570(2003) [PubMed] [Europe PMC] [Abstract]
  15. "Identification and characterization of a novel Pyk2/related adhesion focal tyrosine kinase-associated protein that inhibits alpha-synuclein phosphorylation."J. Biol. Chem. 278:42225-42233(2003) [PubMed] [Europe PMC] [Abstract]
  16. "Pyk2- and Src-dependent tyrosine phosphorylation of PDK1 regulates focal adhesions."Mol. Cell. Biol. 23:8019-8029(2003) [PubMed] [Europe PMC] [Abstract]
  17. "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry."Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003) [PubMed] [Europe PMC] [Abstract]
  18. "Integrin engagement-induced inhibition of human myelopoiesis is mediated by proline-rich tyrosine kinase 2 gene products."Exp. Hematol. 32:365-374(2004) [PubMed] [Europe PMC] [Abstract]
  19. "RAFTK/Pyk2 activation is mediated by trans-acting autophosphorylation in a Src-independent manner."J. Biol. Chem. 279:33315-33322(2004) [PubMed] [Europe PMC] [Abstract]
  20. "Interaction of Pyk2 and PTP-PEST with leupaxin in prostate cancer cells."Am. J. Physiol. 292:C2288-C2296(2007) [PubMed] [Europe PMC] [Abstract]
  21. "Ephrin-A1 stimulates migration of CD8+CCR7+ T lymphocytes."Eur. J. Immunol. 37:2326-2336(2007) [PubMed] [Europe PMC] [Abstract]
  22. "Antitumor activity and pharmacology of a selective focal adhesion kinase inhibitor, PF-562,271." Cancer Res. 68:1935-1944(2008) [PubMed] [Europe PMC] [Abstract]
  23. "Proline-rich tyrosine kinase 2 (Pyk2) promotes proliferation and invasiveness of hepatocellular carcinoma cells through c-Src/ERK activation."Carcinogenesis 29:2096-2105(2008) [PubMed] [Europe PMC] [Abstract]
  24. "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
  25. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  26. "The atypical Rho GTPase Wrch1 collaborates with the nonreceptor tyrosine kinases Pyk2 and Src in regulating cytoskeletal dynamics."Mol. Cell. Biol. 28:1802-1814(2008) [PubMed] [Europe PMC] [Abstract]
  27. "Nonmuscle myosin light-chain kinase mediates neutrophil transmigration in sepsis-induced lung inflammation by activating beta2 integrins."Nat. Immunol. 9:880-886(2008) [PubMed] [Europe PMC] [Abstract]
  28. "A Pyk2-Vav1 complex is recruited to beta3-adhesion sites to initiate Rho activation."Biochem. J. 420:49-56(2009) [PubMed] [Europe PMC] [Abstract]
  29. "Identification of small molecule inhibitors of proline-rich tyrosine kinase 2 (Pyk2) with osteogenic activity in osteoblast cells."Bioorg. Med. Chem. Lett. 19:4924-4928(2009) [PubMed] [Europe PMC] [Abstract]
  30. "Pyk2 mediates endothelin-1 signaling via p130Cas/BCAR3 cascade and regulates human glomerular mesangial cell adhesion and spreading."J. Cell. Physiol. 219:45-56(2009) [PubMed] [Europe PMC] [Abstract]
  31. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  32. "Recruitment of Pyk2 to SHPS-1 signaling complex is required for IGF-I-dependent mitogenic signaling in vascular smooth muscle cells."Cell. Mol. Life Sci. 67:3893-3903(2010) [PubMed] [Europe PMC] [Abstract]
  33. "Pyk2 inhibition of p53 as an adaptive and intrinsic mechanism facilitating cell proliferation and survival."J. Biol. Chem. 285:1743-1753(2010) [PubMed] [Europe PMC] [Abstract]
  34. "The T cell receptor-mediated phosphorylation of Pyk2 tyrosines 402 and 580 occurs via a distinct mechanism than other receptor systems."J. Leukoc. Biol. 87:691-701(2010) [PubMed] [Europe PMC] [Abstract]
  35. "T cell receptor activation leads to two distinct phases of Pyk2 activation and actin cytoskeletal rearrangement in human T cells."Mol. Immunol. 47:1665-1674(2010) [PubMed] [Europe PMC] [Abstract]
  36. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  37. "Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of nephrocystin-1 to control targeting to monocilia."J. Biol. Chem. 286:14237-14245(2011) [PubMed] [Europe PMC] [Abstract]
  38. "Proline-rich tyrosine kinase 2 (Pyk2) promotes cell motility of hepatocellular carcinoma through induction of epithelial to mesenchymal transition."PLoS ONE 6:E18878-E18878(2011) [PubMed] [Europe PMC] [Abstract]
  39. "Focal adhesion kinase-related protein tyrosine kinase Pyk2 in T-cell activation and function."Immunol. Res. 31:267-282(2005) [PubMed] [Europe PMC] [Abstract]
  40. "Targeting Pyk2 for therapeutic intervention."Expert Opin. Ther. Targets 14:95-108(2010) [PubMed] [Europe PMC] [Abstract]
  41. "Cellular functions of FAK kinases: insight into molecular mechanisms and novel functions."J. Cell Sci. 123:1007-1013(2010) [PubMed] [Europe PMC] [Abstract]
  42. "Redox sensitive Pyk2 as a target for therapeutics in breast cancer."Front. Biosci. 16:568-577(2011) [PubMed] [Europe PMC] [Abstract]
  43. "Trifluoromethylpyrimidine-based inhibitors of proline-rich tyrosine kinase 2 (PYK2): structure-activity relationships and strategies for the elimination of reactive metabolite formation." Bioorg. Med. Chem. Lett. 18:6071-6077(2008) [PubMed] [Europe PMC] [Abstract]
  44. "Crystal structures of free and ligand-bound focal adhesion targeting domain of Pyk2."Biochem. Biophys. Res. Commun. 383:347-352(2009) [PubMed] [Europe PMC] [Abstract]
  45. "Sulfoximine-substituted trifluoromethylpyrimidine analogs as inhibitors of proline-rich tyrosine kinase 2 (PYK2) show reduced hERG activity."Bioorg. Med. Chem. Lett. 19:3253-3258(2009) [PubMed] [Europe PMC] [Abstract]
  46. "Structural characterization of proline-rich tyrosine kinase 2 (PYK2) reveals a unique (DFG-out) conformation and enables inhibitor design."J. Biol. Chem. 284:13193-13201(2009) [PubMed] [Europe PMC] [Abstract]
  47. "Structure of protein tyrosine kinase 2 beta (PTK2B) kinase domain."Submitted (JUL-2011) to the PDB data bank
  48. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]