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Tubulin Polymerization Promoting Protein (TPPP)

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TPPP1; p25alpha; p25; TPPP/p25; Brain Specific Protein p25 Alpha; 25 kDa brain-specific protein

Tubulin Polymerization Promoting Protein (TPPP)
CCC A993
HGNC 24164
MGI 1920198
PubMed 15474353
RGD 1310121
UniProt O94811
Wiki TPPP

Seki et al. (1999) cloned p25 from a neuroblastoma cDNA library. The deduced 219-amino acid protein has a calculated molecular mass of about 24 kD. Human and bovine p25 share 90% amino acid identity. In contrast to the brain-specific expression of bovine p25, RT-PCR detected human p25 in all tissues examined.Martin et al. (2002) found that p24 associated with Gsk3 in microtubules from adult rat brain. p24 bound Gsk3 and inhibited its kinase activity at low magnesium concentrations. p24 was a poor substrate for Gsk3, but it could be phosphorylated by other protein kinases. Bovine Tppp inhibited mitotic spindle assembly and nuclear envelope breakdown following injection into Drosophila embryos. It did not affect other cellular events.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA993Hu01 Recombinant Tubulin Polymerization Promoting Protein (TPPP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA993Hu01 Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP) WB,IHC,ICC/IF
MAA993Hu21 Monoclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP) WB; IHC; ICC; IP.
Assay Kits SEA993Hu ELISA Kit for Tubulin Polymerization Promoting Protein (TPPP) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA993Mu01 Recombinant Tubulin Polymerization Promoting Protein (TPPP) Positive Control; Immunogen; SDS-PAGE; WB.
RPA993Mu02 Recombinant Tubulin Polymerization Promoting Protein (TPPP) Positive Control; Immunogen; SDS-PAGE; WB.
EPA993Mu61 Eukaryotic Tubulin Polymerization Promoting Protein (TPPP) Positive Control; Immunogen; SDS-PAGE; WB.
APA993Mu61 Active Tubulin Polymerization Promoting Protein (TPPP) Cell culture; Activity Assays.
Antibodies PAA993Mu01 Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP) WB; IHC; ICC; IP.
PAA993Mu02 Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP) WB; IHC; ICC; IP.
Assay Kits SEA993Mu ELISA Kit for Tubulin Polymerization Promoting Protein (TPPP) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Tubulin Polymerization Promoting Protein (TPPP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Tubulin Polymerization Promoting Protein (TPPP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Tubulin Polymerization Promoting Protein (TPPP) ELISA Kit Customized Service Offer
  1. "A novel human gene whose product shares significant homology with the bovine brain-specific protein p25 on chromosome 5p15.3."J. Hum. Genet. 44:121-122(1999) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "p25alpha Stimulates alpha-synuclein aggregation and is co-localized with aggregated alpha-synuclein in alpha-synucleinopathies."J. Biol. Chem. 280:5703-5715(2005) [PubMed] [Europe PMC] [Abstract]
  4. "Tubulin polymerization promoting proteins (TPPPs): members of a new family with distinct structures and functions."Biochemistry 45:13818-13826(2006) [PubMed] [Europe PMC] [Abstract]
  5. "Interaction of TPPP/p25 protein with glyceraldehyde-3-phosphate dehydrogenase and their co-localization in Lewy bodies."FEBS Lett. 580:5807-5814(2006) [PubMed] [Europe PMC] [Abstract]
  6. "The phosphorylation of p25/TPPP by LIM kinase 1 inhibits its ability to assemble microtubules."Exp. Cell Res. 313:4091-4106(2007) [PubMed] [Europe PMC] [Abstract]
  7. "Phosphorylation blocks the activity of tubulin polymerization-promoting protein (TPPP): identification of sites targeted by different kinases."J. Biol. Chem. 282:29531-29539(2007) [PubMed] [Europe PMC] [Abstract]
  8. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  9. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]