Cyclophilin A (CYPA)

[Edit]

PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein

Cyclophilin A (CYPA)

Cyclophilin A, also called Peptidyl-prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis- and trans- proline residues critical for proper folding of proteins. Cyclophilin is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity. The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity and prevent incorporation of cyclophilin into viral particles. The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin, making it a useful inhibitor for studying calcineurin activity.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA979Hu01 Recombinant Cyclophilin A (CYPA) Positive Control; Immunogen; SDS-PAGE; WB.
CPA979Hu21 OVA Conjugated Cyclophilin A (CYPA) Immunogen; SDS-PAGE; WB.
Antibodies PAA979Hu01 Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP; FCM
MAA979Hu22 Monoclonal Antibody to Cyclophilin A (CYPA) WB,IHC,ICC/IF
LAA979Hu71 Biotin-Linked Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC.
LAA979Hu81 FITC-Linked Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IF.
PAA979Hu08 Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
MAA979Hu21 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC
LAA979Hu72 Biotin-Linked Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC.
CAA979Mi22 Anti-Cyclophilin A (CYPA) Monoclonal Antibody Loading Control of WB.
MAA979Hu23 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
MAA979Hu24 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
MAA979Hu25 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
Assay Kits SEA979Hu ELISA Kit for Cyclophilin A (CYPA) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA979Mu01 Recombinant Cyclophilin A (CYPA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA979Mu02 Recombinant Cyclophilin A (CYPA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA979Mu01 Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
LAA979Mu71 Biotin-Linked Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC.
LAA979Mu81 FITC-Linked Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IF.
PAA979Mu02 Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
MAA979Mu21 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
CAA979Mi22 Anti-Cyclophilin A (CYPA) Monoclonal Antibody Loading Control of WB.
MAA979Mu22 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC
Assay Kits SEA979Mu ELISA Kit for Cyclophilin A (CYPA) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA979Mu High Sensitive ELISA Kit for Cyclophilin A (CYPA) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA979Ra01 Recombinant Cyclophilin A (CYPA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA979Ra01 Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
LAA979Ra71 Biotin-Linked Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC.
LAA979Ra81 FITC-Linked Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IF.
MAA979Ra21 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
MAA979Ra24 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
MAA979Ra23 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
MAA979Ra22 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
MAA979Ra25 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
MAA979Ra27 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
MAA979Ra29 Monoclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
Assay Kits SEA979Ra ELISA Kit for Cyclophilin A (CYPA) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rhesus monkey (Simian)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA979Si01 Recombinant Cyclophilin A (CYPA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA979Si01 Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
LAA979Si71 Biotin-Linked Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Cyclophilin A (CYPA) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cyclophilin A (CYPA) ELISA Kit Customized Service Offer

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA979Po01 Recombinant Cyclophilin A (CYPA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA979Po01 Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
LAA979Po71 Biotin-Linked Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Cyclophilin A (CYPA) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cyclophilin A (CYPA) ELISA Kit Customized Service Offer

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA979Bo01 Recombinant Cyclophilin A (CYPA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA979Bo01 Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IP.
LAA979Bo71 Biotin-Linked Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC.
LAA979Bo81 FITC-Linked Polyclonal Antibody to Cyclophilin A (CYPA) WB; IHC; ICC; IF.
Assay Kits n/a CLIA Kit for Cyclophilin A (CYPA) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cyclophilin A (CYPA) ELISA Kit Customized Service Offer
  1. "Complementary DNA for human T-cell cyclophilin."EMBO J. 6:947-950(1987) [PubMed] [Europe PMC] [Abstract]
  2. "Characterization of the human cyclophilin gene and of related processed pseudogenes."Eur. J. Biochem. 190:477-482(1990) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence of human chromosome 7." Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Identification of cyclophilin A from human decidual and placental tissue in the first trimester of pregnancy."Hum. Reprod. 10:1305-1310(1995) [PubMed] [Europe PMC] [Abstract]
  8. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
  9. , Submitted (DEC-2008) to UniProtKB
  10. , Submitted (NOV-2005) to UniProtKB
  11. "Human and Escherichia coli cyclophilins: sensitivity to inhibition by the immunosuppressant cyclosporin A correlates with a specific tryptophan residue."Biochemistry 30:2306-2310(1991) [PubMed] [Europe PMC] [Abstract]
  12. "Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B."Cell 73:1067-1078(1993) [PubMed] [Europe PMC] [Abstract]
  13. "Cyclophilin A is secreted by a vesicular pathway in vascular smooth muscle cells."Circ. Res. 98:811-817(2006) [PubMed] [Europe PMC] [Abstract]
  14. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  17. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  18. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  19. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  20. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  21. "Structure of human cyclophilin and its binding site for cyclosporin A determined by X-ray crystallography and NMR spectroscopy."Nature 353:276-279(1991) [PubMed] [Europe PMC] [Abstract]
  22. "Crystal structure of recombinant human T-cell cyclophilin A at 2.5-A resolution."Proc. Natl. Acad. Sci. U.S.A. 88:9483-9487(1991) [PubMed] [Europe PMC] [Abstract]
  23. "X-ray structure of a decameric cyclophilin-cyclosporin crystal complex."Nature 361:91-94(1993) [PubMed] [Europe PMC] [Abstract]
  24. "X-ray structure of a monomeric cyclophilin A-cyclosporin A crystal complex at 2.1-A resolution."J. Mol. Biol. 234:1119-1130(1993) [PubMed] [Europe PMC] [Abstract]
  25. "Crystal structure implies that cyclophilin predominantly catalyzes the trans to cis isomerization."Biochemistry 35:7356-7361(1996) [PubMed] [Europe PMC] [Abstract]
  26. "Crystal structure of cyclophilin A complexed with a binding site peptide from the HIV-1 capsid protein."Protein Sci. 6:2297-2307(1997) [PubMed] [Europe PMC] [Abstract]
  27. "X-ray structures and analysis of 11 cyclosporin derivatives complexed with cyclophilin A."J. Mol. Biol. 283:435-449(1998) [PubMed] [Europe PMC] [Abstract]
  28. "Crystal structure of calcineurin-cyclophilin-cyclosporin shows common but distinct recognition of immunophilin-drug complexes."Proc. Natl. Acad. Sci. U.S.A. 99:12037-12042(2002) [PubMed] [Europe PMC] [Abstract]
  29. "Crystal structure of human calcineurin complexed with cyclosporin A and human cyclophilin."Proc. Natl. Acad. Sci. U.S.A. 99:13522-13526(2002) [PubMed] [Europe PMC] [Abstract]
  30. "Solution structure of the cyclosporin A/cyclophilin complex by NMR."Nature 361:88-91(1993) [PubMed] [Europe PMC] [Abstract]
  31. "The NMR solution conformation of unligated human cyclophilin A."J. Mol. Biol. 272:64-81(1997) [PubMed] [Europe PMC] [Abstract]
  32. "Acetylation regulates cyclophilin A catalysis, immunosuppression and HIV isomerization."Nat. Chem. Biol. 6:331-337(2010) [PubMed] [Europe PMC] [Abstract]