Osteopontin (OPN)

[Edit]

SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

Osteopontin (OPN)

Osteopontin (OPN), is a secreted multifunctional glyco-phosphoprotein with roles in bone metabolism, immune regulation, tissue remodeling , cell survival, and tumor progression . Gene structure and chromosomal location identify OPN as a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family that also includes BSP,BMP1,DSPP, ENAM and MEPE. Murine OPN issynthesized as a 294 amino acid (aa) precursor protein with a predicted 16 aa signal peptide and a highly unusual mature protein sequence, containing 68 acidic aa and 23 potential Ser/Thr phosphorylation sites. Although the predicted molecular weight of OPN is 31 kDa,phosphorylation and N- and O-glycosylation may allow it to appear as large as 75 kDa. Variability in post-translational modifications can influence the activity of OPN.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA899Hu02 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
EPA899Hu61 Eukaryotic Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
APA899Hu61 Active Osteopontin (OPN) Cell culture; Activity Assays.
EPA899Hu62 Eukaryotic Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAA899Hu22 Monoclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
LAA899Hu71 Biotin-Linked Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC.
PAA899Hu02 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP; FCM
PAA899Hu06 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
Assay Kits SEA899Hu ELISA Kit for Osteopontin (OPN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA899Hu CLIA Kit for Osteopontin (OPN) Chemiluminescent immunoassay for Antigen Detection.
AEA899Hu ELISA Kit for Anti-Osteopontin Antibody (Anti-OPN) Enzyme-linked immunosorbent assay for Antibody Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA899Mu01 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA899Mu02 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
EPA899Mu61 Eukaryotic Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
APA899Mu61 Active Osteopontin (OPN) Cell culture; Activity Assays.
RPA899Mu03 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA899Mu01 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
PAA899Mu02 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
PAA899Mu06 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
Assay Kits SEA899Mu ELISA Kit for Osteopontin (OPN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA899Mu CLIA Kit for Osteopontin (OPN) Chemiluminescent immunoassay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA899Ra01 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
EPA899Ra61 Eukaryotic Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
APA899Ra61 Active Osteopontin (OPN) Cell culture; Activity Assays.
Antibodies PAA899Ra01 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
MAA899Ra21 Monoclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
PAA899Ra06 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
Assay Kits SEA899Ra ELISA Kit for Osteopontin (OPN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA899Rb01 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA899Rb51 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
MAA899Rb21 Monoclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
Assay Kits SEA899Rb ELISA Kit for Osteopontin (OPN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rhesus monkey (Simian)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA899Si01 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA899Si01 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
Assay Kits SEA899Si ELISA Kit for Osteopontin (OPN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA899Ca01 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA899Ca02 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA899Ca01 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
LAA899Ca71 Biotin-Linked Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC.
Assay Kits SEA899Ca ELISA Kit for Osteopontin (OPN) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA899Ca High Sensitive ELISA Kit for Osteopontin (OPN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA899Po01 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA899Po01 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
MAA899Po21 Monoclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
MAA899Po22 Monoclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
MAA899Po23 Monoclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
MAA899Po24 Monoclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
Assay Kits SEA899Po ELISA Kit for Osteopontin (OPN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA899Bo01 Recombinant Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
EPA899Bo61 Eukaryotic Osteopontin (OPN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA899Bo01 Polyclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
MAA899Bo21 Monoclonal Antibody to Osteopontin (OPN) WB; IHC; ICC; IP.
Assay Kits SEA899Bo ELISA Kit for Osteopontin (OPN) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "The cDNA and derived amino acid sequence for human osteopontin."Nucleic Acids Res. 17:3306-3306(1989) [PubMed] [Europe PMC] [Abstract]
  2. "cDNA cloning, mRNA distribution and heterogeneity, chromosomal location, and RFLP analysis of human osteopontin (OPN)."Genomics 7:491-502(1990) [PubMed] [Europe PMC] [Abstract]
  3. "Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily."Proc. Natl. Acad. Sci. U.S.A. 89:426-430(1992) [PubMed] [Europe PMC] [Abstract]
  4. "Genomic organization of the human osteopontin gene: exclusion of the locus from a causative role in the pathogenesis of dentinogenesis imperfecta type II."Genomics 27:155-160(1995) [PubMed] [Europe PMC] [Abstract]
  5. "Cloning and characterization of the human osteopontin gene and its promoter."Biochem. J. 303:255-262(1994) [PubMed] [Europe PMC] [Abstract]
  6. "Expression of osteopontin in human glioma. Its correlation with the malignancy."Lab. Invest. 72:55-63(1995) [PubMed] [Europe PMC] [Abstract]
  7. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
  9. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  10. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin."Biochem. Biophys. Res. Commun. 184:859-864(1992) [PubMed] [Europe PMC] [Abstract]
  12. "Purification of a human milk protein closely similar to tumor-secreted phosphoproteins and osteopontin."Biochim. Biophys. Acta 996:43-48(1989) [PubMed] [Europe PMC] [Abstract]
  13. "Post-translationally modified residues of native human osteopontin are located in clusters: identification of 36 phosphorylation and five O-glycosylation sites and their biological implications."Biochem. J. 390:285-292(2005) [PubMed] [Europe PMC] [Abstract]
  14. "Phosphoproteomic analysis of the human pituitary."Pituitary 9:109-120(2006) [PubMed] [Europe PMC] [Abstract]
  15. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  16. "Secreted kinase phosphorylates extracellular proteins that regulate biomineralization."Science 336:1150-1153(2012) [PubMed] [Europe PMC] [Abstract]
  17. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
  18. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]